Protein and mRNA Expression Levels of Interleukin-17A, -17F and -22 in Blood and Skin Samples of Patients with Mycosis Fungoides.


Journal

Acta dermato-venereologica
ISSN: 1651-2057
Titre abrégé: Acta Derm Venereol
Pays: Sweden
ID NLM: 0370310

Informations de publication

Date de publication:
18 Nov 2020
Historique:
pubmed: 11 11 2020
medline: 24 6 2021
entrez: 10 11 2020
Statut: epublish

Résumé

This study investigated the expression of interleukin (IL)-17A, -17F and -22 in mycosis fungoides. Blood samples were collected from 50 patients with mycosis fungoides and 50 healthy controls. Skin samples were obtained from 26 patients with mycosis fungoides and 5 healthy controls. Protein levels of IL-17A, -17F and -22 were measured in serum by multiplex enzyme-linked immunosorbent assay, and mRNA expression levels were measured in blood and skin samples by real-time quantitative reverse transcription PCR. Both IL-17A and IL-17F mRNA expression levels were significantly lower in blood of patients with mycosis fungoides in comparison with healthy controls. IL-22 serum levels and expression levels of IL-22 mRNA in skin tissue, were significantly increased in patients with mycosis fungoides in comparison with healthy controls. These results suggest that low levels of IL-17A and IL-17F in mycosis fungoides may be connected to impaired immune surveillance contributing to tumourigenesis. Upregulation of IL-22 may play a role in the establishment of the tumour microenvironment in mycosis fungoides.

Identifiants

pubmed: 33170303
doi: 10.2340/00015555-3688
pmc: PMC9309846
doi:

Substances chimiques

IL17A protein, human 0
IL17F protein, human 0
Interleukin-17 0
Interleukins 0
RNA, Messenger 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

adv00326

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Auteurs

Despoina Papathemeli (D)

2nd Department of Dermatology and Venereology, Cutaneous Lymphoma Outpatient Clinic, Faculty of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.

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Classifications MeSH