Cold-inducible RNA binding protein promotes breast cancer cell malignancy by regulating Cystatin C levels.
Breast Neoplasms
/ genetics
Cell Line
Cell Line, Tumor
Cell Proliferation
Cystatin C
/ genetics
Epithelial Cells
/ cytology
Female
Gene Expression Regulation, Neoplastic
Gene Regulatory Networks
Humans
Mammary Glands, Human
/ metabolism
Protein Binding
Protein Interaction Mapping
RNA, Messenger
/ genetics
RNA-Binding Proteins
/ genetics
Signal Transduction
Survival Analysis
CIRBP
CST3
breast cancer
Journal
RNA (New York, N.Y.)
ISSN: 1469-9001
Titre abrégé: RNA
Pays: United States
ID NLM: 9509184
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
14
05
2020
accepted:
05
11
2020
pubmed:
12
11
2020
medline:
22
4
2021
entrez:
11
11
2020
Statut:
ppublish
Résumé
Cold-inducible RNA binding protein (CIRBP) is a stress-responsive protein that promotes cancer development and inflammation. Critical to most CIRBP functions is its capacity to bind and posttranscriptionally modulate mRNA. However, a transcriptome-wide analysis of CIRBP mRNA targets in cancer has not yet been performed. Here, we use an ex vivo breast cancer model to identify CIRBP targets and mechanisms. We find that CIRBP transcript levels correlate with breast cancer subtype and are an indicator of luminal A/B prognosis. Accordingly, overexpression of CIRBP in nontumoral MCF-10A cells promotes cell growth and clonogenicity, while depletion of CIRBP from luminal A MCF-7 cells has opposite effects. We use RNA immunoprecipitation followed by high-throughput sequencing (RIP-seq) to identify a set of 204 high confident CIRBP targets in MCF-7 cells. About 10% of these showed complementary changes after CIRBP manipulation in MCF-10A and MCF-7 cells, and were highly interconnected with known breast cancer genes. To test the potential of CIRBP-mediated regulation of these targets in breast cancer development, we focused on
Identifiants
pubmed: 33172965
pii: rna.076422.120
doi: 10.1261/rna.076422.120
pmc: PMC7812870
doi:
Substances chimiques
CIRBP protein, human
0
CST3 protein, human
0
Cystatin C
0
RNA, Messenger
0
RNA-Binding Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
190-201Informations de copyright
© 2021 Indacochea et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.
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