Metabolic Checkpoint Aldehyde Dehydrogenases Are Important for Diverting β-Oxidation into 1-Butanol Biosynthesis from Kitchen Waste Oil in Pseudomonas aeruginosa.
1-Butanol
Binding energy
Checkpoint
Forward β-oxidation
Kitchen waste oil
Journal
Applied biochemistry and biotechnology
ISSN: 1559-0291
Titre abrégé: Appl Biochem Biotechnol
Pays: United States
ID NLM: 8208561
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
06
08
2020
accepted:
08
11
2020
pubmed:
13
11
2020
medline:
7
7
2021
entrez:
12
11
2020
Statut:
ppublish
Résumé
1-Butanol (1-BD) is a promising fuel additive which can be biosynthesized via reversed β-oxidation pathway in bacteria. However, heterologous reversed β-oxidation pathway is a carbon chain prolongation process with several genes overexpressed in most of bacterial hosts, leading to low titer of 1-BD and high cost for production. Here we displayed a forward β-oxidation pathway for 1-BD production in a kitchen waste oil (KWO) degrading Pseudomonas aeruginosa PA-3, and we proved that aldehyde dehydrogenase (ALDH) is a checkpoint for diverting metabolic flux into 1-BD biosynthesis. With nitrogen source supplied, titer of 1-BD was increased accompanied with 12 ALDH coding genes transcriptionally promoted to different degrees. At the same time, binding energies of these ALDHs with different length of acyl-CoAs in β-oxidation were calculated to identify their specificities. Based on the above information, ALDH deletions were conducted. We certified that deletion of ALDH8 and ALDH9 led to significant decreased titers of 1-BD. Finally, these two ALDHs were separately overexpressed in PA-3, and titer of 1-BD was promoted to 1.36 g/L at 72 h in shake flask. Totally in this work, we provided a forward β-oxidation pathway for 1-BD production from KWO, and the roles of ALDHs were confirmed.
Identifiants
pubmed: 33180312
doi: 10.1007/s12010-020-03456-x
pii: 10.1007/s12010-020-03456-x
doi:
Substances chimiques
Bacterial Proteins
0
Oils
0
Waste Water
0
1-Butanol
8PJ61P6TS3
Aldehyde Dehydrogenase
EC 1.2.1.3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
730-742Subventions
Organisme : Beijing Municipal Education Commission Technology Plan
ID : KM202011417006
Organisme : Project of Beijing Municipal Commission of Education
ID : KZ201911417049
Organisme : Premium Funding Project for Academic Human Resources Development in Beijing Union University
ID : BPHR2018BZ01
Organisme : China Scholarship Council Project
ID : 201908110070
Références
Mohan, S. V., Nikhil, G., Chiranjeevi, P., Reddy, C. N., Rohit, M., Kumar, A. N., & Sarkar, O. (2016). Waste biorefinery models towards sustainable circular bioeconomy: Critical review and future perspectives. Bioresource Technology, 215, 2–12.
doi: 10.1016/j.biortech.2016.03.130
Sticklen, M. B. (2008). Plant genetic engineering for biofuel production: Towards affordable cellulosic ethanol. Nature Reviews Genetics, 9(6), 433–443.
doi: 10.1038/nrg2336
Wang, M., Liu, L., Fan, L., & Tan, T. (2017). CRISPRi based system for enhancing 1-butanol production in engineered Klebsiella pneumoniae. Process Biochemistry, 56, 139–146.
doi: 10.1016/j.procbio.2017.02.013
Nielsen, D. R., Yoon, S. H., Yuan, C. J., & Prather, K. L. (2010). Metabolic engineering of acetoin and meso-2, 3-butanediol biosynthesis in E. coli. Biotechnology Journal, 5(3), 274–284.
doi: 10.1002/biot.200900279
Nanthagopal, K., Ashok, B., Saravanan, B., Patel, D., Sudarshan, B., & Ramasamy, R. A. (2018). An assessment on the effects of 1-pentanol and 1-butanol as additives with Calophyllum Inophyllum biodiesel. Energy Conversion and Management, 158, 70–80.
doi: 10.1016/j.enconman.2017.12.048
Nawab, S., Wang, N., Ma, X., & Huo, Y.-X. (2020). Genetic engineering of non-native hosts for 1-butanol production and its challenges: A review. Microbial Cell Factories, 19, 1–16.
doi: 10.1186/s12934-020-01337-w
Prapaporn, B. (2007). Applications of microemulsions in cosmetics. Journal of Cosmetic Dermatology, 6(4), 223–228.
doi: 10.1111/j.1473-2165.2007.00337.x
Srinivasan, K., Palanivelu, K., & Gopalakrishnan, A. N. (2007). Recovery of 1-butanol from a model pharmaceutical aqueous waste by pervaporation. Chemical Engineering Science, 62(11), 2905–2914.
doi: 10.1016/j.ces.2007.02.028
Atsumi, S., & Liao, J. C. (2008). Directed evolution of Methanococcus jannaschii citramalate synthase for biosynthesis of 1-propanol and 1-butanol by Escherichia coli. Applied and Environmental Microbiology, 74(24), 7802–7808.
doi: 10.1128/AEM.02046-08
Horn, S. J., Nguyen, Q. D., Westereng, B., Nilsen, P. J., & Eijsink, V. G. (2011). Screening of steam explosion conditions for glucose production from non-impregnated wheat straw. Biomass and Bioenergy, 35(12), 4879–4886.
doi: 10.1016/j.biombioe.2011.10.013
Koutinas, A., Arifeen, N., Wang, R., & Webb, C. (2007). Cereal-based biorefinery development: Integrated enzyme production for cereal flour hydrolysis. Biotechnology and Bioengineering, 97(1), 61–72.
doi: 10.1002/bit.21206
Parajuli, R., Dalgaard, T., Jørgensen, U., Adamsen, A. P. S., Knudsen, M. T., Birkved, M., Gylling, M., & Schjørring, J. K. (2015). Biorefining in the prevailing energy and materials crisis: A review of sustainable pathways for biorefinery value chains and sustainability assessment methodologies. Renewable and Sustainable Energy Reviews, 43, 244–263.
doi: 10.1016/j.rser.2014.11.041
Dong, H., Zhao, C., Zhang, T., Zhu, H., Lin, Z., Tao, W., Zhang, Y., & Li, Y. (2017). A systematically chromosomally engineered Escherichia coli efficiently produces butanol. Metabolic Engineering, 44, 284–292.
doi: 10.1016/j.ymben.2017.10.014
Li, Y., Cui, T., Wang, Y., & Ge, X. (2018). Isolation and characterization of a novel bacterium Pseudomonas aeruginosa for biofertilizer production from kitchen waste oil. RSC Advances, 8(73), 41966–41975.
doi: 10.1039/C8RA09779H
Sun, A., Cheng, Y., Zhang, Y., Zhang, Q., Wang, S., Tian, S., Zou, Y., Hu, K., Ren, J., & Ge, J. (2014). Aldehyde dehydrogenase 2 ameliorates doxorubicin-induced myocardial dysfunction through detoxification of 4-HNE and suppression of autophagy. Journal of Molecular and Cellular Cardiology, 71, 92–104.
doi: 10.1016/j.yjmcc.2014.01.002
Tuck, L. R., Altenbach, K., Ang, T. F., Crawshaw, A. D., Campopiano, D. J., Clarke, D. J., & Marles-Wright, J. (2016). Insight into coenzyme A cofactor binding and the mechanism of acyl-transfer in an acylating aldehyde dehydrogenase from Clostridium phytofermentans. Scientific Reports, 6(1), 22108.
doi: 10.1038/srep22108
Zarzycki, J., Sutter, M., Cortina, N. S., Erb, T. J., & Kerfeld, C. A. (2017). In vitro characterization and concerted function of three core enzymes of a glycyl radical enzyme-associated bacterial microcompartment. Scientific Reports, 7(1), 1–12.
doi: 10.1038/srep42757
Kovach, M. E., Elzer, P. H., Hill, D. S., Robertson, G. T., Farris, M. A., Roop II, R. M., & Peterson, K. M. (1995). Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene, 166(1), 175–176.
doi: 10.1016/0378-1119(95)00584-1
Wang, X., Zhang, L., Xi, B., Sun, W., Xia, X., Zhu, C., He, X., Li, M., Yang, T., & Wang, P. (2015). Biogas production improvement and C/N control by natural clinoptilolite addition into anaerobic co-digestion of Phragmites australis, feces and kitchen waste. Bioresource Technology, 180, 192–199.
doi: 10.1016/j.biortech.2014.12.023
Wang, M., Fan, L., & Tan, T. (2014). 1-Butanol production from glycerol by engineered Klebsiella pneumoniae. RSC Advances, 4(101), 57791–57798.
doi: 10.1039/C4RA09016K
Zhang, J., Zong, W., Hong, W., Zhang, Z.-T., & Wang, Y. (2018). Exploiting endogenous CRISPR-Cas system for multiplex genome editing in Clostridium tyrobutyricum and engineer the strain for high-level butanol production. Metabolic Engineering, 47, 49–59.
doi: 10.1016/j.ymben.2018.03.007
Sampson, E. M., & Bobik, T. A. (2008). Microcompartments for B
doi: 10.1128/JB.01925-07
Jorda, J., Lopez, D., Wheatley, N. M., & Yeates, T. O. (2013). Using comparative genomics to uncover new kinds of protein-based metabolic organelles in bacteria. Protein Science, 22(2), 179–195.
doi: 10.1002/pro.2196
Bobik, T. A., Havemann, G. D., Busch, R. J., Williams, D. S., & Aldrich, H. C. (1999). The propanediol utilization (pdu) operon of Salmonella enterica Serovar Typhimurium LT2 includes genes necessary for formation of polyhedral organelles involved in coenzyme B
doi: 10.1128/JB.181.19.5967-5975.1999
Petit, E., LaTouf, W. G., Coppi, M. V., Warnick, T. A., Currie, D., Romashko, I., Deshpande, S., Haas, K., Alvelo-Maurosa, J. G., & Wardman, C. (2013). Involvement of a bacterial microcompartment in the metabolism of fucose and rhamnose by Clostridium phytofermentans. PLoS One, 8(1), e54337.
doi: 10.1371/journal.pone.0054337
Chen, C., Sun, N., Li, D., Long, S., Tang, X., Xiao, G., & Wang, L. (2018). Optimization and characterization of biosurfactant production from kitchen waste oil using Pseudomonas aeruginosa. Environmental Science and Pollution Research, 25(15), 14934–14943.
doi: 10.1007/s11356-018-1691-1
Tetko, I. V., & Bruneau, P. (2004). Application of ALOGPS to predict 1-octanol/water distribution coefficients, logP, and logD, of AstraZeneca in-house database. Journal of Pharmaceutical Sciences, 93(12), 3103–3110.
doi: 10.1002/jps.20217
Mills, P., Magnusson, B., & Cross, S. (2004). The effect of region of application on absorption of ethanol and hexanol through canine skin. Research in Veterinary Science, 76(1), 37–41.
doi: 10.1016/S0034-5288(03)00142-5
Ishige, T., Tani, A., Sakai, Y., & Kato, N. (2000). Long-chain aldehyde dehydrogenase that participates in n-alkane utilization and wax ester synthesis in Acinetobacter sp. strain M-1. Applied and Environmental Microbiology, 66(8), 3481–3486.
doi: 10.1128/AEM.66.8.3481-3486.2000