A quick and sensitive diagnostic tool for detection of Maize streak virus.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
12 11 2020
12 11 2020
Historique:
received:
02
06
2020
accepted:
29
10
2020
entrez:
13
11
2020
pubmed:
14
11
2020
medline:
26
3
2021
Statut:
epublish
Résumé
Maize streak virus disease (MSVD), caused by Maize streak virus (MSV; genus Mastrevirus), is one of the most severe and widespread viral diseases that adversely reduces maize yield and threatens food security in Africa. An effective control and management of MSVD requires robust and sensitive diagnostic tests capable of rapid detection of MSV. In this study, a loop-mediated isothermal amplification (LAMP) assay was designed for the specific detection of MSV. This test has shown to be highly specific and reproducible and able to detect MSV in as little as 10 fg/µl of purified genomic DNA obtained from a MSV-infected maize plant, a sensitivity 10
Identifiants
pubmed: 33184360
doi: 10.1038/s41598-020-76612-2
pii: 10.1038/s41598-020-76612-2
pmc: PMC7661706
doi:
Substances chimiques
DNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
19633Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R005397/1 (Training Voucher)
Pays : United Kingdom
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