High-affinity antigen association to cationic liposomes via coiled coil-forming peptides induces a strong antigen-specific CD4
Adjuvants, Immunologic
/ administration & dosage
Animals
CD4 Antigens
/ administration & dosage
CD4-Positive T-Lymphocytes
/ immunology
Cell Proliferation
Drug Compounding
/ methods
Immunogenicity, Vaccine
Liposomes
Mice
Mice, Transgenic
Models, Animal
Peptides
/ chemistry
Protein Conformation, alpha-Helical
Structure-Activity Relationship
Antigen association
CD4 T-cell
Coiled coil peptide
Liposomes
Vaccination
Journal
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
ISSN: 1873-3441
Titre abrégé: Eur J Pharm Biopharm
Pays: Netherlands
ID NLM: 9109778
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
11
08
2020
revised:
05
11
2020
accepted:
08
11
2020
pubmed:
15
11
2020
medline:
21
9
2021
entrez:
14
11
2020
Statut:
ppublish
Résumé
Liposomes are widely investigated as vaccine delivery systems, but antigen loading efficiency can be low. Moreover, adsorbed antigen may rapidly desorb under physiological conditions. Encapsulation of antigens overcomes the latter problem but results in significant antigen loss during preparation and purification of the liposomes. Here, we propose an alternative attachment method, based on a complementary heterodimeric coiled coil peptide pair pepK and pepE. PepK was conjugated to cholesterol (yielding CPK) and pepE was covalently linked to model antigen OVA323 (yielding pepE-OVA323). CPK was incorporated in the lipid bilayer of cationic liposomes (180 nm in size). Antigen was associated more efficiently to functionalized liposomes (Kd 166 nM) than to cationic liposomes (Kd not detectable). In vivo co-localization of antigen and liposomes was strongly increased upon CPK-functionalization (35% -> 80%). CPK-functionalized liposomes induced 5-fold stronger CD4
Identifiants
pubmed: 33188929
pii: S0939-6411(20)30328-3
doi: 10.1016/j.ejpb.2020.11.005
pii:
doi:
Substances chimiques
Adjuvants, Immunologic
0
CD4 Antigens
0
Liposomes
0
Peptides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
96-105Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.