Key role of CCR2-expressing macrophages in a mouse model of low back pain and radiculopathy.
CCR2
CX3CR1
Clodronate
Dorsal root ganglion
INCB3344
MCP-1
Macrophage
Radicular pain
Journal
Brain, behavior, and immunity
ISSN: 1090-2139
Titre abrégé: Brain Behav Immun
Pays: Netherlands
ID NLM: 8800478
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
24
08
2020
revised:
12
10
2020
accepted:
11
11
2020
pubmed:
17
11
2020
medline:
28
5
2021
entrez:
16
11
2020
Statut:
ppublish
Résumé
Chronic low back pain is a common condition, with high societal costs and often ineffectual treatments. Communication between macrophages/monocytes (MØ) and sensory neurons has been implicated in various preclinical pain models. However, few studies have examined specific MØ subsets, although distinct subtypes may play opposing roles. This study used a model of low back pain/radiculopathy involving direct local inflammation of the dorsal root ganglia (DRG). Reporter mice were employed that had distinct fluorescent labels for two key MØ subsets: CCR2-expressing (infiltrating pro-inflammatory) MØ, and CX3CR1-expressing (resident) macrophages. We observed that local DRG inflammation induced pain behaviors in mice, including guarding behavior and mechanical hypersensitivity, similar to the previously described rat model. The increase in MØ in the inflamed DRG was dominated by increases in CCR2
Identifiants
pubmed: 33197543
pii: S0889-1591(20)32391-6
doi: 10.1016/j.bbi.2020.11.015
pmc: PMC7758110
mid: NIHMS1648444
pii:
doi:
Substances chimiques
Ccr2 protein, mouse
0
Chemokine CCL2
0
Receptors, CCR2
0
Clodronic Acid
0813BZ6866
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
556-567Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR068989
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS045594
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS113243
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
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