Steroid precursors, steroids, neuroactive steroids, and neurosteroids concentrations in serum and saliva of healthy neonatal heifer Holstein calves.
brain
cattle
consciousness
encephalopathy
neurosteroids
Journal
Journal of veterinary internal medicine
ISSN: 1939-1676
Titre abrégé: J Vet Intern Med
Pays: United States
ID NLM: 8708660
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
08
05
2020
revised:
21
10
2020
accepted:
23
10
2020
pubmed:
18
11
2020
medline:
16
6
2021
entrez:
17
11
2020
Statut:
ppublish
Résumé
Persistence of high neurosteroid concentrations in blood is associated with neonatal encephalopathy and septicemia in foals. This has not been investigated in calves. To determine concentrations of steroid compounds in serum and saliva within the first 48 hours after birth in healthy neonatal calves, identify potential markers for disease, and investigate the association between serum steroid compounds concentrations in calves and their respective dams within 2 hours after birth. Twelve healthy neonatal heifer Holstein calves and their dams. Prospective study. Serum and saliva were collected from calves at 2, 6, 24, and 48 hours after birth. Steroid compounds were analyzed using liquid chromatography-mass spectrometry. A nonlinear regression model was used to determine half-lives of the neurosteroids. Serum concentrations of neurosteroids between the cows and calves were compared using the Wilcoxon signed rank test. Half-lives (95% confidence intervals) of dehydroepiandrosterone (DHEA) and 17α,20α-dihydroxyprogesterone in calf serum were 2.9 (2.1, 4.3), and 2.1 (1.3, 3.0) hours, respectively. Pregnanediol in saliva had a half-life (95% confidence interval) of 24.5 (14.2, 66.5) hours. Serum DHEA (1718.7 ± 2313 vs 57.7 ± 44) and 17α,20α-dihydroxyprogesterone (207.8 ± 198.2 vs 43.5 ± 33.5) concentrations respectively were higher (P < .05) in calves compared to cows. Dehydroepiandrosterone, 17α,20α-dihydroxyprogesterone, and pregnanediol could be potential markers of disease in neonatal heifer calves with unexplained failure to thrive or encephalopathy. However, because of the wide 95% confidence interval of the half-life, pregnanediol in saliva might not be a potential marker.
Sections du résumé
BACKGROUND
BACKGROUND
Persistence of high neurosteroid concentrations in blood is associated with neonatal encephalopathy and septicemia in foals. This has not been investigated in calves.
OBJECTIVES
OBJECTIVE
To determine concentrations of steroid compounds in serum and saliva within the first 48 hours after birth in healthy neonatal calves, identify potential markers for disease, and investigate the association between serum steroid compounds concentrations in calves and their respective dams within 2 hours after birth.
ANIMALS
METHODS
Twelve healthy neonatal heifer Holstein calves and their dams.
METHODS
METHODS
Prospective study. Serum and saliva were collected from calves at 2, 6, 24, and 48 hours after birth. Steroid compounds were analyzed using liquid chromatography-mass spectrometry. A nonlinear regression model was used to determine half-lives of the neurosteroids. Serum concentrations of neurosteroids between the cows and calves were compared using the Wilcoxon signed rank test.
RESULTS
RESULTS
Half-lives (95% confidence intervals) of dehydroepiandrosterone (DHEA) and 17α,20α-dihydroxyprogesterone in calf serum were 2.9 (2.1, 4.3), and 2.1 (1.3, 3.0) hours, respectively. Pregnanediol in saliva had a half-life (95% confidence interval) of 24.5 (14.2, 66.5) hours. Serum DHEA (1718.7 ± 2313 vs 57.7 ± 44) and 17α,20α-dihydroxyprogesterone (207.8 ± 198.2 vs 43.5 ± 33.5) concentrations respectively were higher (P < .05) in calves compared to cows.
CONCLUSIONS AND CLINICAL IMPORTANCE
CONCLUSIONS
Dehydroepiandrosterone, 17α,20α-dihydroxyprogesterone, and pregnanediol could be potential markers of disease in neonatal heifer calves with unexplained failure to thrive or encephalopathy. However, because of the wide 95% confidence interval of the half-life, pregnanediol in saliva might not be a potential marker.
Identifiants
pubmed: 33201530
doi: 10.1111/jvim.15957
pmc: PMC7694825
doi:
Substances chimiques
Neurosteroids
0
Steroids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2767-2775Subventions
Organisme : Gifts from anonymous donors towards the Equine and Comparative Neurology Research Group
Informations de copyright
© 2020 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
Références
Eur Child Adolesc Psychiatry. 2014 Jun;23(6):485-98
pubmed: 24043498
J Vet Intern Med. 2020 Nov;34(6):2767-2775
pubmed: 33201530
J Vet Intern Med. 2017 Nov;31(6):1892-1899
pubmed: 28865106
J Am Vet Med Assoc. 1976 Feb 1;168(3):223-5
pubmed: 175041
Clin Biochem Rev. 2009 Feb;30(1):19-34
pubmed: 19224008
Trends Pharmacol Sci. 2016 Jul;37(7):543-561
pubmed: 27156439
Equine Vet J. 2011 Jul;43(4):487-93
pubmed: 21496072
J Reprod Fertil Suppl. 1991;44:609-17
pubmed: 1795303
Adv Biochem Psychopharmacol. 1992;47:87-92
pubmed: 1354922
J Endocrinol. 1975 Jul;66(1):61-70
pubmed: 1172518
Eur J Neurosci. 1998 Sep;10(9):2905-12
pubmed: 9758160
Equine Vet J. 2017 Jan;49(1):45-50
pubmed: 26538009
Psychoneuroendocrinology. 1998 Nov;23(8):963-87
pubmed: 9924747
Animal. 2014 May;8 Suppl 1:70-81
pubmed: 24703103
Front Endocrinol (Lausanne). 2011 Oct 04;2:44
pubmed: 22654809
Equine Vet J Suppl. 2012 Feb;(41):109-12
pubmed: 22594038
Ann N Y Acad Sci. 1995 Dec 29;771:257-72
pubmed: 8597405
Equine Vet J. 2017 Mar;49(2):207-210
pubmed: 26728952
Equine Vet J. 2013 Nov;45(6):661-5
pubmed: 23600660
Pharmacol Biochem Behav. 2006 Aug;84(4):644-55
pubmed: 16962651
J Vet Intern Med. 2019 Sep;33(5):2286-2293
pubmed: 31489708
Vitam Horm. 2018;108:145-174
pubmed: 30029725
J Reprod Fertil Suppl. 1975 Oct;(23):691-4
pubmed: 1060866
N Z Vet J. 2020 Jan;68(1):65-68
pubmed: 31554484
Am J Vet Res. 1977 Feb;38(2):263-8
pubmed: 65932
FASEB J. 1992 Mar;6(6):2311-22
pubmed: 1347506
J Perinatol. 2017 May;37(5):591-595
pubmed: 28102853
J Steroid Biochem Mol Biol. 2019 Apr;188:77-85
pubmed: 30557606
J Dairy Sci. 2004 Jun;87(6):1713-8
pubmed: 15453483
Theriogenology. 2018 Dec;122:130-136
pubmed: 30265893
Equine Vet J Suppl. 1988 Sep;(5):41-3
pubmed: 9118085
Proc Annu Meet U S Anim Health Assoc. 1973;(77):67-72
pubmed: 4534745
Animals (Basel). 2017 Sep 05;7(9):
pubmed: 28872596
Vet J. 2017 Jul;225:42-49
pubmed: 28720298
Acta Vet Scand. 2001;42(1):151-9
pubmed: 11455895
Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13284-9
pubmed: 9789080
Prog Neuropsychopharmacol Biol Psychiatry. 2005 Feb;29(2):169-92
pubmed: 15694225
Anal Bioanal Chem. 2010 Jan;396(2):799-808
pubmed: 19915825