Scanning Electron Microscopy-based palynological characterization of selected Euphorbiaceae taxa colonized in Pakistan.
Euphorbiaceae
P/E ratio
colpi
equatorial diameter
exine
intine
palynological characterization
polar diameter
pollens
Journal
Microscopy research and technique
ISSN: 1097-0029
Titre abrégé: Microsc Res Tech
Pays: United States
ID NLM: 9203012
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
revised:
20
10
2020
received:
11
09
2020
accepted:
28
10
2020
pubmed:
19
11
2020
medline:
19
8
2021
entrez:
18
11
2020
Statut:
ppublish
Résumé
The present study was carried out on scanning electron microscopic-based palynological characterization of selected 20 Euphorbiaceae taxa. In this study, important pollen qualitative and quantitative features of selected Euphorbiaceae species such as polar diameter, equatorial diameter, P/E ratio, sculpturing of pollen, exine thickness, intine thickness, presence or absence of colpi and length of colpi, and pollen fertility were examined. Remarkable variations in these pollen characters had been observed among these species. In both polar and equatorial views, Riccinus communis L. exhibited the highest pollen size (polar = 264.1 μm, Equatorial = 270 μm), while Acalypha wilkesiana Mull Arg. showed lowest pollen size (Polar = 17 μm, Equatorial = 18.5 μm). Most of the pollen varied from oblate to spheroidal in shape. With regard to the P/E ratio, Sapium sebiferum L. Roxb showed the highest ratio while Bischofia javanica Blume showed minimum, that is, 1.18 and 0.85, respectively. Intine and exine thickness also varied. Most of the pollen grains were monade. Tricolporate, tricolpate, monocolpate, pentaporate, and polyporate pollen aperture types have been observed. Most of the plants showed regular, reticulate, and echinate pollen sculpturing. Pollen fertility also varied from species to species but most of the plant showed pollen fertility between 70 and 80%. Through this work, it has been concluded that quantitative and qualitative pollen features are helpful at the specific level as well as the generic level and can provide a fruitful taxonomic solution.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
850-859Informations de copyright
© 2020 Wiley Periodicals LLC.
Références
Ahmad, K., Shaheen, N., Ahmad, M., & Khan, M. A. (2010). Pollen fertility estimation of some sub-tropical flora of Pakistan. African Journal of Biotechnology, 9(49), 8313-8317.
Bijekar, S., & Gayatri, M. C. (2014). Ethanomedicinal properties of Euphorbiaceae family-A comprehensive review. International Journal of Phytomedicine, 6, 144-150.
El-Ghazaly, G. A., & Chaudhary, R. (1993). Pollen morphology of some species of the genus Euphorbia L. Review of Palaeobotany and Palynology, 78(3), 293-319.
Khalil, A. T., Shinwari, Z. K., Qaiser, M., & Marwat, K. B. (2014). Phyto-therapeutic claims about plants belonging to Pakistan; an ethnomedicinal review. Pakistan Journal of Botany, 46(3), 1137-1144.
Khan, R., Abidin, S. Z. U., Ahmed, M., Zafar, M., Liu, J., & Amina, H. (2017). Palyno-Morphological Characteristics of gymnopsperm flora of Pakistan and its taxonomic implications wuth LM and SEM methods. Microscopy Research and Technique, 18, 21.
Kumar G.P, Chaturvedi A. Ethnobotanical observations of Euphorbiaceae species from Vidarbha region, Maharashtra, India. Ethnobotanical Leaflets,. 2010;14 674-680.
Mvine, J. T., & Van damme, T. (2011). Why do Euphorbiaceae tick as medicinal Plants? A review of Euphorbiaceae family and its medicinal features. Journal of Medicinal Plant Research, 5(5), 652-662.
Ndam, L. M., Mih, A. M., Tening, A. S., Fongod, A. G. N., Temenue, N. A., & Fujii, Y. (2015). Foliar micromorphology of Euphorbia golondrina L.C. Wheeler (Euphorbiaceae) From Cameroon. International Journal of Current research, 7(07), 18261-18267.
Perveen, A., & Qaiser, M. (2005). Pollen Flora of Pakistan-XL VII. Euphorbiaceae. Pakistan Journal of Botany, 37(4), 785-796.
Punt, W. (1961). Pollen morphology of Euphorbiaceae with special references to taxonomy. Wentia, 7, 1-116.
Radcliffe-Smith, A. Karachi, Pakistan: University of Karachi,; (1986). Euphorbiaceae. In E. Nasir & S. I. Ali (Eds.), Flora of Pakistan (Vol. 172, p. 1170).
Ramzan, S., Shaheen, S., Hussain, K., Sharifi-Rad, J., Khan, M. A., Harun, N., … Khan, F. (2019). Implication of palynological techniques for the authentication of adulterated drugs traded with the same name in different herbal markets of district Lahore, Pakistan. Microscopy Research and Technique, 82(1), 2.
Saad, S. I., & El-Ghazaly, G. (1988). Pollen Morphology of some species of Euphorbiaceae. Grana, 27(3), 165-175.
Shaheen, S., Jaffer, M., Ilyas, S., Hanif, U., Khan, F., Siddique, Z., … Ishtiaq, S. (2017). Study of systematic application of morphopalynological characterization of medicinal plants. Transylvanian Review, 25(19).22-25.
Wurdack, K. J., Hoffman, P., Samuel, R., Bruijn, A. D., Bank, M. V. D., & Chase, M. W. (2004). Molecular phylogenetic analysis of phyllanthaceae using plastid RBCL DNA sequences1. American Journal of Botany, 9(11), 1882-1900.
Zafar, M., Ahmad, M., Khan, M. A., Sultana, S., Jan, G., Ahmad, F., … Marwat, S. K. (2011). Chemotaxonomic clarification of pharmaceutically important species of Cyperus L. African Journal of Pharmacy & Pharmacology, 51(1), 67-75.