Inactivation of the three GGA genes in HeLa cells partially compromises lysosomal enzyme sorting.
AP-1
CI-MPR
GGA1
GGA2
GGA3
cathepsin D sorting
Journal
FEBS open bio
ISSN: 2211-5463
Titre abrégé: FEBS Open Bio
Pays: England
ID NLM: 101580716
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
29
10
2020
accepted:
12
11
2020
pubmed:
19
11
2020
medline:
15
12
2021
entrez:
18
11
2020
Statut:
ppublish
Résumé
The Golgi-localized, gamma-ear containing, ADP-ribosylation factor-binding proteins (GGAs 1, 2, and 3) are multidomain proteins that bind mannose 6-phosphate receptors (MPRs) at the Golgi and play a role, along with adaptor protein complex 1 (AP-1), in the sorting of newly synthesized lysosomal hydrolases to the endolysosomal system. However, the relative importance of the two types of coat proteins in this process is still unclear. Here, we report that inactivation of all three GGA genes in HeLa cells decreased the sorting efficiency of cathepsin D from 97% to 73% relative to wild-type, with marked redistribution of the cation-independent MPR from peripheral punctae to the trans-Golgi network. In comparison, GNPTAB
Identifiants
pubmed: 33206455
doi: 10.1002/2211-5463.13040
pmc: PMC7876502
doi:
Substances chimiques
Adaptor Protein Complex 1
0
Adaptor Proteins, Vesicular Transport
0
GGA adaptor proteins
0
GGA2 protein, human
0
Transferases (Other Substituted Phosphate Groups)
EC 2.7.8.-
GNPTAB protein, human
EC 2.7.8.15
CTSD protein, human
EC 3.4.23.5
Cathepsin D
EC 3.4.23.5
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
367-374Subventions
Organisme : NCI NIH HHS
ID : R01 CA008759
Pays : United States
Informations de copyright
© 2020 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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