Cytoskeletal Protein Zyxin Inhibits the Activity of Genes Responsible for Embryonic Stem Cell Status.
Animals
Body Patterning
/ genetics
Cell Differentiation
/ genetics
Cytoskeletal Proteins
/ metabolism
Cytoskeleton
/ metabolism
Embryonic Stem Cells
/ metabolism
Gene Expression Regulation, Developmental
/ genetics
HEK293 Cells
Humans
Kruppel-Like Factor 4
Morphogenesis
Neural Plate
/ metabolism
Octamer Transcription Factor-3
/ genetics
Xenopus laevis
/ metabolism
Zebrafish
/ metabolism
Zyxin
/ metabolism
KLF4
Nanog
Vent
Ybx1
Zyxin
actin cytoskeleton regulation
genes responsible for stem cells status
pou5f3
regulation of mRNA stability
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
17 11 2020
17 11 2020
Historique:
received:
25
02
2020
revised:
14
09
2020
accepted:
26
10
2020
entrez:
18
11
2020
pubmed:
19
11
2020
medline:
9
11
2021
Statut:
ppublish
Résumé
Zyxin is a cytoskeletal LIM-domain protein that regulates actin cytoskeleton assembly and gene expression. In the present work, we find that zyxin downregulation in Xenopus laevis embryos reduces the expression of numerous genes that regulate cell differentiation, but it enhances that of several genes responsible for embryonic stem cell status, specifically klf4, pou5f3.1, pou5f3.2, pou5f3.3, and vent2.1/2. For pou5f3 family genes (mammalian POU5F1/OCT4 homologs), we show that this effect is the result of mRNA stabilization due to complex formation with the Y-box protein Ybx1. When bound to Ybx1, zyxin interferes with the formation of these complexes, thereby stimulating pou5f3 mRNA degradation. In addition, in zebrafish embryos and human HEK293 cells, zyxin downregulation increases mRNA levels of the pluripotency genes KLF4, NANOG, and POU5F1/OCT4. Our findings indicate that zyxin may play a role as a switch among morphogenetic cell movement, differentiation, and embryonic stem cell status.
Identifiants
pubmed: 33207197
pii: S2211-1247(20)31385-1
doi: 10.1016/j.celrep.2020.108396
pii:
doi:
Substances chimiques
Cytoskeletal Proteins
0
KLF4 protein, human
0
Kruppel-Like Factor 4
0
Octamer Transcription Factor-3
0
Zyxin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108396Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.