Gene expression profiling reveals insights into infant immunological and febrile responses to group B meningococcal vaccine.
Animals
Blood Chemical Analysis
Diphtheria-Tetanus-Pertussis Vaccine
/ adverse effects
Female
Fever
/ blood
Gene Expression Profiling
Haemophilus Vaccines
/ adverse effects
Host-Pathogen Interactions
/ genetics
Humans
Immunity
/ genetics
Incidence
Infant
Male
Meningococcal Infections
/ prevention & control
Meningococcal Vaccines
/ adverse effects
Mice
Mice, Inbred C57BL
Microarray Analysis
Pneumococcal Vaccines
/ adverse effects
Poliovirus Vaccine, Inactivated
/ adverse effects
Proteome
/ analysis
Transcriptome
Vaccination
/ adverse effects
Vaccines, Conjugate
/ adverse effects
paediatrics
proteomics
systems biology
transcriptomics
vaccines
Journal
Molecular systems biology
ISSN: 1744-4292
Titre abrégé: Mol Syst Biol
Pays: England
ID NLM: 101235389
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
26
07
2020
revised:
06
10
2020
accepted:
08
10
2020
entrez:
19
11
2020
pubmed:
20
11
2020
medline:
26
8
2021
Statut:
ppublish
Résumé
Neisseria meningitidis is a major cause of meningitis and septicaemia. A MenB vaccine (4CMenB) was licensed by the European Medicines Agency in January 2013. Here we describe the blood transcriptome and proteome following infant immunisations with or without concomitant 4CMenB, to gain insight into the molecular mechanisms underlying post-vaccination reactogenicity and immunogenicity. Infants were randomised to receive control immunisations (PCV13 and DTaP-IPV-Hib) with or without 4CMenB at 2 and 4 months of age. Blood gene expression and plasma proteins were measured prior to, then 4 h, 24 h, 3 days or 7 days post-vaccination. 4CMenB vaccination was associated with increased expression of ENTPD7 and increased concentrations of 4 plasma proteins: CRP, G-CSF, IL-1RA and IL-6. Post-vaccination fever was associated with increased expression of SELL, involved in neutrophil recruitment. A murine model dissecting the vaccine components found the concomitant regimen to be associated with increased gene perturbation compared with 4CMenB vaccine alone with enhancement of pathways such as interleukin-3, -5 and GM-CSF signalling. Finally, we present transcriptomic profiles predictive of immunological and febrile responses following 4CMenB vaccine.
Identifiants
pubmed: 33210468
doi: 10.15252/msb.20209888
pmc: PMC7674973
doi:
Substances chimiques
13-valent pneumococcal vaccine
0
Diphtheria-Tetanus-Pertussis Vaccine
0
Haemophilus Vaccines
0
Meningococcal Vaccines
0
Pneumococcal Vaccines
0
Poliovirus Vaccine, Inactivated
0
Proteome
0
Vaccines, Conjugate
0
diphtheria-tetanus-five component acellular pertussis-inactivated poliomyelitis -Haemophilus influenzae type b conjugate vaccine
0
Banques de données
GEO
['GSE131929', 'GSE132199']
ClinicalTrials.gov
['NCT02080559']
Types de publication
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
e9888Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/M007693/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 090532/Z/09/Z
Pays : United Kingdom
Organisme : Department of Health
Pays : United Kingdom
Informations de copyright
© 2020 The Authors. Published under the terms of the CC BY 4.0 license.
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