Mms19 promotes spindle microtubule assembly in Drosophila neural stem cells.
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
01
06
2020
accepted:
13
10
2020
revised:
03
12
2020
pubmed:
20
11
2020
medline:
2
2
2021
entrez:
19
11
2020
Statut:
epublish
Résumé
Mitotic divisions depend on the timely assembly and proper orientation of the mitotic spindle. Malfunctioning of these processes can considerably delay mitosis, thereby compromising tissue growth and homeostasis, and leading to chromosomal instability. Loss of functional Mms19 drastically affects the growth and development of mitotic tissues in Drosophila larvae and we now demonstrate that Mms19 is an important factor that promotes spindle and astral microtubule (MT) growth, and MT stability and bundling. Mms19 function is needed for the coordination of mitotic events and for the rapid progression through mitosis that is characteristic of neural stem cells. Surprisingly, Mms19 performs its mitotic activities through two different pathways. By stimulating the mitotic kinase cascade, it triggers the localization of the MT regulatory complex TACC/Msps (Transforming Acidic Coiled Coil/Minispindles, the homolog of human ch-TOG) to the centrosome. This activity of Mms19 can be rescued by stimulating the mitotic kinase cascade. However, other aspects of the Mms19 phenotypes cannot be rescued in this way, pointing to an additional mechanism of Mms19 action. We provide evidence that Mms19 binds directly to MTs and that this stimulates MT stability and bundling.
Identifiants
pubmed: 33211700
doi: 10.1371/journal.pgen.1008913
pii: PGENETICS-D-20-00873
pmc: PMC7714366
doi:
Substances chimiques
Drosophila Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008913Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Dev Cell. 2007 Mar;12(3):467-74
pubmed: 17336911
Cell Div. 2011 Apr 08;6(1):8
pubmed: 21477279
Nat Cell Biol. 2001 Jul;3(7):643-9
pubmed: 11433296
Cell Div. 2010 Sep 15;5:24
pubmed: 20840796
J Cell Biol. 2008 Jul 28;182(2):289-300
pubmed: 18663142
Science. 2012 Jul 13;337(6091):195-9
pubmed: 22678362
Cell Tissue Res. 1993 Sep;273(3):583-98
pubmed: 8402833
J Cell Biol. 2007 Jun 18;177(6):995-1004
pubmed: 17576796
Curr Biol. 2009 Sep 29;19(18):1549-54
pubmed: 19682904
Mol Cell. 2007 Mar 23;25(6):839-50
pubmed: 17386261
Development. 2012 Dec 1;139(23):4297-310
pubmed: 23132240
Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):2158-63
pubmed: 24469800
Oncogene. 2015 May 21;34(21):2777-89
pubmed: 25065591
Curr Biol. 2008 Dec 23;18(24):R1146-9
pubmed: 19108775
Genes Dev. 2006 Dec 15;20(24):3464-74
pubmed: 17182871
Science. 2012 Jul 13;337(6091):243-5
pubmed: 22678361
Mol Cell. 2010 Aug 27;39(4):632-40
pubmed: 20797633
Nat Commun. 2015 Nov 16;6:8879
pubmed: 26568519
PLoS One. 2012;7(6):e38011
pubmed: 22723842
PLoS Genet. 2010 Mar 12;6(3):e1000876
pubmed: 20300654
J Cell Biol. 2002 Feb 4;156(3):437-51
pubmed: 11827981
Cold Spring Harb Protoc. 2010 Jul 01;2010(7):pdb.prot5460
pubmed: 20647364
Nat Rev Mol Cell Biol. 2004 Jun;5(6):481-92
pubmed: 15173827
Mol Biol Cell. 2015 Apr 15;26(8):1452-62
pubmed: 25694445
J Cell Sci. 2014 Oct 1;127(Pt 19):4111-22
pubmed: 25128564
Biomolecules. 2019 Jan 15;9(1):
pubmed: 30650622
J Cell Sci. 2012 Nov 1;125(Pt 21):4951-61
pubmed: 23277534
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Nature. 2003 Jul 10;424(6945):228-32
pubmed: 12853965
J Cell Biol. 2003 Dec 8;163(5):1089-98
pubmed: 14657233
Curr Biol. 2008 Aug 26;18(16):1256-61
pubmed: 18718761
Nat Protoc. 2013 May;8(5):958-65
pubmed: 23598446
J Biol Chem. 2012 Dec 21;287(52):43351-8
pubmed: 23150669
Trends Neurosci. 2001 May;24(5):251-4
pubmed: 11311363
J Biol Chem. 2009 Nov 20;284(47):32651-61
pubmed: 19778897
Curr Biol. 2015 Jun 1;25(11):1542-50
pubmed: 26004761
J Biol Chem. 2010 Jul 9;285(28):21849-57
pubmed: 20444701
Mol Gen Genet. 1979 Nov;176(3):351-9
pubmed: 392238
J Cell Biol. 2014 Mar 31;204(7):1111-21
pubmed: 24687279
Development. 2018 Jan 25;145(2):
pubmed: 29361561
Nat Commun. 2015 Nov 17;6:8894
pubmed: 26573328
J Cell Sci. 2020 Jun 23;133(12):
pubmed: 32501288
Genes Dev. 1998 Jun 15;12(12):1847-57
pubmed: 9637686
Antimicrob Agents Chemother. 2014 Jul;58(7):3934-41
pubmed: 24777105
Genes Dev. 2003 Oct 15;17(20):2520-5
pubmed: 14561775
Dev Cell. 2009 Jul;17(1):134-41
pubmed: 19619498
Cold Spring Harb Protoc. 2013 Oct 01;2013(10):970-7
pubmed: 24086057
Genes Dev. 1998 Feb 1;12(3):370-81
pubmed: 9450931
Dev Cell. 2006 Aug;11(2):147-57
pubmed: 16890155
J Cell Sci. 2011 Mar 1;124(Pt 5):706-17
pubmed: 21285248
Cold Spring Harb Protoc. 2011 Sep 01;2011(9):
pubmed: 21880819
Curr Biol. 2019 Sep 23;29(18):2993-3005.e9
pubmed: 31495584
Int Rev Cytol. 2008;265:111-58
pubmed: 18275887