3D Bioprinting of Human Adipose-Derived Stem Cells and Their Tenogenic Differentiation in Clinical-Grade Medium.
3D bioprinting
adipose-derived stem cells
alginate
collagen
nanocellulose
scleraxis
tendon tissue engineering
tenogenic differentiation
xenogenic-free
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
18 Nov 2020
18 Nov 2020
Historique:
received:
28
08
2020
revised:
11
11
2020
accepted:
13
11
2020
entrez:
21
11
2020
pubmed:
22
11
2020
medline:
5
3
2021
Statut:
epublish
Résumé
Defining the best combination of cells and biomaterials is a key challenge for the development of tendon tissue engineering (TE) strategies. Adipose-derived stem cells (ASCs) are ideal candidates for this purpose. In addition, controlled cell-based products adherent to good manufacturing practice (GMP) are required for their clinical scale-up. With this aim, in this study, ASC 3D bioprinting and GMP-compliant tenogenic differentiation were investigated. In detail, primary human ASCs were embedded within a nanofibrillar-cellulose/alginate bioink and 3D-bioprinted into multi-layered square-grid matrices. Bioink viscoelastic properties and scaffold ultrastructural morphology were analyzed by rheology and scanning electron microscopy (SEM). The optimal cell concentration for printing among 3, 6 and 9 × 10
Identifiants
pubmed: 33218011
pii: ijms21228694
doi: 10.3390/ijms21228694
pmc: PMC7698777
pii:
doi:
Substances chimiques
Culture Media
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Horizon 2020 Framework Programme
ID : direct action of JRC (2013/743/EU Council Decision of 3 December 2013 establishing the specific programme implementing Horizon 2020)
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