Novel spore lytic enzyme from a Bacillus phage leading to spore killing.
Bacillus phage
spore killing
spore lytic enzyme
Journal
Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
31
07
2020
revised:
19
10
2020
accepted:
20
10
2020
entrez:
22
11
2020
pubmed:
23
11
2020
medline:
30
7
2021
Statut:
ppublish
Résumé
Bacterial spores maintain metabolic dormancy and have high resistance to external pressure. Germination requires degradation of the spore cortex and the participation of germination-specific cortex-lytic enzymes (GSLEs). Previously reported GSLEs have been identified in bacteria and facilitate germination. In this study, we have characterized a novel spore lytic enzyme, Ply67, from Bacillus pumilus phage vB_BpuM_BpSp. Ply67 had a similar cortex-lytic activity to GSLEs but disrupted the inner membranes (IMs) of spores, leading to spore killing rather than germination. The amino acid sequence of the complete protein, Ply67
Identifiants
pubmed: 33220860
pii: S0141-0229(20)30191-5
doi: 10.1016/j.enzmictec.2020.109698
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Amidohydrolases
EC 3.5.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109698Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.