An Approach to Monitor Exocytosis in White Adipocytes.

3T3-L1 Cells Adipocytes, White / metabolism Animals Cell Membrane / genetics Exocytosis / genetics Mice Patch-Clamp Techniques / methods
3T3-L1 adipocytes Adipocyte differentiation Cell isolation Exocytosis Membrane capacitance Patch-clamp Stromal vascular cell fraction White adipocyte Whole-cell configuration

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2021
Historique:
entrez: 22 11 2020
pubmed: 23 11 2020
medline: 26 3 2021
Statut: ppublish

Résumé

Exocytosis, the fusion of vesicles with the plasma membrane, can be measured with the patch-clamp technique as increases in membrane capacitance. Here we provide detailed information on how to monitor white adipocyte exocytosis using this method. We describe how to isolate the stromal vascular fraction of cells (SVF) within adipose tissue and how to differentiate SVF and cultured 3T3-L1 cells into adipocytes suitable for patch-clamp studies. We also give detailed protocols of how to record and analyze exocytosis in the differentiated cells.

Identifiants

DOI: 10.1007/978-1-0716-1044-2_14 PMID: 33222137
pubmed: 33222137
doi: 10.1007/978-1-0716-1044-2_14
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

203-222

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Auteurs

Ali M Komai (AM)

Department of Physiology/Metabolic Physiology, Institute of Neuroscience and Physiology, The Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden.

Man Mohan Shrestha (MM)

Department of Physiology/Metabolic Physiology, Institute of Neuroscience and Physiology, The Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden.

Saliha Musovic (S)

Department of Physiology/Metabolic Physiology, Institute of Neuroscience and Physiology, The Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden.

Charlotta S Olofsson (CS)

Department of Physiology/Metabolic Physiology, Institute of Neuroscience and Physiology, The Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden. charlotta.olofsson@gu.se.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Fibroblastes › Cellules 3T3 › Cellules Swiss 3T3 › Cellules 3T3-L1 › An Approach to Monitor Exocytosis in White Adipocytes.
questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Adipocytes › Adipocytes blancs › An Approach to Monitor Exocytosis in White Adipocytes.
questionsmedicales.fr › Eucaryotes › Animaux › An Approach to Monitor Exocytosis in White Adipocytes.
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › An Approach to Monitor Exocytosis in White Adipocytes.
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Exocytose › An Approach to Monitor Exocytosis in White Adipocytes.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › An Approach to Monitor Exocytosis in White Adipocytes.
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Techniques de patch-clamp › An Approach to Monitor Exocytosis in White Adipocytes.