Zirconium(IV)-IMAC Revisited: Improved Performance and Phosphoproteome Coverage by Magnetic Microparticles for Phosphopeptide Affinity Enrichment.

IMAC MOAC affinity enrichment cell signaling functional proteomics magnetic particles mass spectrometry protein phosphorylation

Journal

Journal of proteome research
ISSN: 1535-3907
Titre abrégé: J Proteome Res
Pays: United States
ID NLM: 101128775

Informations de publication

Date de publication:
01 01 2021
Historique:
pubmed: 24 11 2020
medline: 22 6 2021
entrez: 23 11 2020
Statut: ppublish

Résumé

Phosphopeptide enrichment is an essential step in large-scale, quantitative phosphoproteomics by mass spectrometry. Several phosphopeptide affinity enrichment techniques exist, such as immobilized metal-ion affinity chromatography (IMAC) and metal oxide affinity chromatography (MOAC). We compared zirconium(IV) IMAC (Zr-IMAC) magnetic microparticles to more commonly used titanium(IV) IMAC (Ti-IMAC) and TiO

Identifiants

pubmed: 33226818
doi: 10.1021/acs.jproteome.0c00508
doi:

Substances chimiques

Ferric Compounds 0
Phosphopeptides 0
Zirconium C6V6S92N3C
Titanium D1JT611TNE

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

453-462

Auteurs

Ignacio Arribas Diez (I)

Department of Biochemistry & Molecular Biology and VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, Odense M DK-5230, Denmark.

Ireshyn Govender (I)

NextGen Health, Council for Scientific and Industrial Research, Pretoria 0184, South Africa.

Previn Naicker (P)

NextGen Health, Council for Scientific and Industrial Research, Pretoria 0184, South Africa.

Stoyan Stoychev (S)

NextGen Health, Council for Scientific and Industrial Research, Pretoria 0184, South Africa.
ReSyn Biosciences, Pretoria 1610, Gauteng, South Africa.

Justin Jordaan (J)

ReSyn Biosciences, Pretoria 1610, Gauteng, South Africa.
Rhodes University, Grahamstown 6139, South Africa.

Ole N Jensen (ON)

Department of Biochemistry & Molecular Biology and VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, Odense M DK-5230, Denmark.

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Classifications MeSH