Specific and sensitive detection of Influenza A virus using a biotin-coated nanoparticle enhanced immunomagnetic assay.
Biotin-nanoparticle
Diagnostics
Immunoassay
Influenza A virus
Magnetic bead
Nucleoprotein
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
received:
01
10
2020
accepted:
17
11
2020
revised:
04
11
2020
pubmed:
25
11
2020
medline:
18
3
2022
entrez:
24
11
2020
Statut:
ppublish
Résumé
This study reports the development of a sensitive magnetic bead-based enzyme-linked immunoassay (MELISA) for the pan-reactive detection of the Influenza A virus. The assay combines immunomagnetic beads and biotin-nanoparticle-based detection to quantify a highly conserved viral nucleoprotein in virus lysates. At the capture step, monoclonal antibody-coated magnetic microbeads were used to bind and concentrate the nucleoprotein in samples. The colorimetric detection signal was amplified using biotinylated silica nanoparticles (NP). These nanoparticles were functionalized on the surface with short DNA spacers bearing biotin groups by an automated supported synthesis method performed on nano-on-micro assemblies with a DNA/RNA synthesizer. A biotin-nanoparticle and immunomagnetic bead-based assay was developed. We succeeded in detecting Influenza A viruses directly in the lysis buffer supplemented with 10% saliva to simulate the clinical context. The biotin-nanoparticle amplification step enabled detection limits as low as 3 × 10
Identifiants
pubmed: 33230699
doi: 10.1007/s00216-020-03081-x
pii: 10.1007/s00216-020-03081-x
pmc: PMC7682758
doi:
Substances chimiques
Antibodies, Monoclonal
0
Biotin
6SO6U10H04
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
265-276Informations de copyright
© 2020. Springer-Verlag GmbH Germany, part of Springer Nature.
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