Distinct Structures and Dynamics of Chromatosomes with Different Human Linker Histone Isoforms.
Cryo-EM
NMR
chromatin structure
chromatosome
chromatosome dynamics
chromatosome structure
linker histone isoform
linker histone tail
nucleosome
single-chain antibody
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
07 01 2021
07 01 2021
Historique:
received:
21
05
2020
revised:
21
09
2020
accepted:
27
10
2020
pubmed:
26
11
2020
medline:
28
1
2021
entrez:
25
11
2020
Statut:
ppublish
Résumé
The repeating structural unit of metazoan chromatin is the chromatosome, a nucleosome bound to a linker histone, H1. There are 11 human H1 isoforms with diverse cellular functions, but how they interact with the nucleosome remains elusive. Here, we determined the cryoelectron microscopy (cryo-EM) structures of chromatosomes containing 197 bp DNA and three different human H1 isoforms, respectively. The globular domains of all three H1 isoforms bound to the nucleosome dyad. However, the flanking/linker DNAs displayed substantial distinct dynamic conformations. Nuclear magnetic resonance (NMR) and H1 tail-swapping cryo-EM experiments revealed that the C-terminal tails of the H1 isoforms mainly controlled the flanking DNA orientations. We also observed partial ordering of the core histone H2A C-terminal and H3 N-terminal tails in the chromatosomes. Our results provide insights into the structures and dynamics of the chromatosomes and have implications for the structure and function of chromatin.
Identifiants
pubmed: 33238161
pii: S1097-2765(20)30772-3
doi: 10.1016/j.molcel.2020.10.038
pmc: PMC7796963
mid: NIHMS1645348
pii:
doi:
Substances chimiques
Histones
0
Nucleosomes
0
Protein Isoforms
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
166-182.e6Subventions
Organisme : Intramural NIH HHS
ID : ZIA BC010808
Pays : United States
Informations de copyright
Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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