Rodolfo Ghirlando

Affiliations

Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520.

Publications (36)

Allosteric control of hemoglobin S fiber formation by oxygen and its relation to the pathophysiology of sickle cell disease.

Proceedings of the National Academy of Sciences of the United States of America

Avec: Eric R Henry , Troy Cellmer , Emily B Dunkelberger , Belhu Metaferia , James Hofrichter , Quan Li , David Ostrowski , Rodolfo Ghirlando , John M Louis , Stéphane Moutereau , Frédéric Galactéros , Swee Lay Thein , Pablo Bartolucci , William A Eaton

Abrogation of prenucleation, transient oligomerization of the Huntingtin exon 1 protein by human profilin I.

Proceedings of the National Academy of Sciences of the United States of America

Avec: Alberto Ceccon , Vitali Tugarinov , Rodolfo Ghirlando , G Marius Clore

Modulation of the monomer-dimer equilibrium and catalytic activity of SARS-CoV-2 main protease by a transition-state analog inhibitor.

Communications biology

Avec: Nashaat T Nashed , Annie Aniana , Rodolfo Ghirlando , Sai Chaitanya Chiliveri , John M Louis

Transposase N-terminal phosphorylation and asymmetric transposon ends inhibit piggyBac transposition in mammalian cells.

Nucleic acids research

Avec: Wentian Luo , Alison B Hickman , Pavol Genzor , Rodolfo Ghirlando , Christopher M Furman , Anna Menshikh , Astrid Haase , Fred Dyda , Matthew H Wilson

Visualizing the active site oxyanion loop transition upon ensitrelvir binding and transient dimerization of SARS-CoV-2 main protease.

Journal of molecular biology

Avec: Andrey Kovalevsky , Annie Aniana , Leighton Coates , Rodolfo Ghirlando , Nashaat T Nashed , John M Louis

Structural basis of tRNA recognition by the widespread OB fold.

Nature communications

Avec: Aline Umuhire Juru , Rodolfo Ghirlando , Jinwei Zhang

Probing initial transient oligomerization events facilitating Huntingtin fibril nucleation at atomic resolution by relaxation-based NMR.

Proceedings of the National Academy of Sciences of the United States of America

Avec: Samuel A Kotler , Vitali Tugarinov , Thomas Schmidt , Alberto Ceccon , David S Libich , Rodolfo Ghirlando , Charles D Schwieters , G Marius Clore

Molecular architecture of a cylindrical self-assembly at human centrosomes.

Nature communications

Avec: Tae-Sung Kim , Liang Zhang , Jong Il Ahn , Lingjun Meng , Yang Chen , Eunhye Lee , Jeong Kyu Bang , Jung Mi Lim , Rodolfo Ghirlando , Lixin Fan , Yun-Xing Wang , Bo Yeon Kim , Jung-Eun Park , Kyung S Lee

Hybrid Thermophilic/Mesophilic Enzymes Reveal a Role for Conformational Disorder in Regulation of Bacterial Enzyme I.

Journal of molecular biology

Avec: Rochelle R Dotas , Trang T Nguyen , Charles E Stewart , Rodolfo Ghirlando , Davit A Potoyan , Vincenzo Venditti

Architectural basis for cylindrical self-assembly governing Plk4-mediated centriole duplication in human cells.

Communications biology

Avec: Jong Il Ahn , Liang Zhang , Harsha Ravishankar , Lixin Fan , Klara Kirsch , Yan Zeng , Lingjun Meng , Jung-Eun Park , Hye-Yeoung Yun , Rodolfo Ghirlando , Buyong Ma , David Ball , Bonsu Ku , Ruth Nussinov , Jeremy D Schmit , William F Heinz , Seung Jun Kim , Tatiana Karpova , Yun-Xing Wang , Kyung S Lee

Reversible phase separation of ESCRT protein ALIX through tyrosine phosphorylation.

Science advances

Avec: Ruben D Elias , Yingqi Zhu , Qi Su , Rodolfo Ghirlando , Jin Zhang , Lalit Deshmukh

Insights into the mechanism of SARS-CoV-2 main protease autocatalytic maturation from model precursors.

Communications biology

Avec: Annie Aniana , Nashaat T Nashed , Rodolfo Ghirlando , Leighton Coates , Daniel W Kneller , Andrey Kovalevsky , John M Louis

Effects of SARS-CoV-2 Main Protease Mutations at Positions L50, E166, and L167 Rendering Resistance to Covalent and Noncovalent Inhibitors.

Journal of medicinal chemistry

Avec: Andrey Kovalevsky , Annie Aniana , Rodolfo Ghirlando , Leighton Coates , Victoria N Drago , Lauren Wear , Oksana Gerlits , Nashaat T Nashed , John M Louis

Probing transient excited states of the bacterial cell division regulator MinE by relaxation dispersion NMR spectroscopy.

Proceedings of the National Academy of Sciences of the United States of America

Avec: Mengli Cai , Ying Huang , Yang Shen , Min Li , Michiyo Mizuuchi , Rodolfo Ghirlando , Kiyoshi Mizuuchi , G Marius Clore

Bismaleimide cross-linked anthrax toxin forms functional octamers with high specificity in tumor targeting.

Protein science : a publication of the Protein Society

Avec: Elyse S Fischer , Warren A Campbell , Shihui Liu , Rodolfo Ghirlando , Rasem J Fattah , Thomas H Bugge , Stephen H Leppla

Quantitative NMR Study of Insulin-Degrading Enzyme Using Amyloid-β and HIV-1 p6 Elucidates Its Chaperone Activity.

Biochemistry

Avec: Bhargavi Ramaraju , Spencer L Nelson , Wenwei Zheng , Rodolfo Ghirlando , Lalit Deshmukh

ALS Variants of Annexin A11's Proline-Rich Domain Impair Its S100A6-Mediated Fibril Dissolution.

ACS chemical neuroscience

Avec: Aman Shihora , Ruben D Elias , John A Hammond , Rodolfo Ghirlando , Lalit Deshmukh

HIV-1 integrase assembles multiple species of stable synaptic complex intasomes that are active for concerted DNA integration in vitro.

Journal of molecular biology

Avec: Min Li , Renbin Yang , Xuemin Chen , Huaibin Wang , Rodolfo Ghirlando , Emilios K Dimitriadis , Robert Craigie

Structural details of helix-mediated TDP-43 C-terminal domain multimerization.

bioRxiv : the preprint server for biology

Avec: Azamat Rizuan , Jayakrishna Shenoy , Priyesh Mohanty , Patricia M S Dos Passos , José F Mercado Ortiz , Leanna Bai , Renjith Viswanathan , Szu-Huan Wang , Victoria Johnson , Lohany D Mamede , Yuna M Ayala , Rodolfo Ghirlando , Jeetain Mittal , Nicolas L Fawzi

Characterization of alternate encounter assemblies of SARS-CoV-2 main protease.

The Journal of biological chemistry

Avec: Annie Aniana , Nashaat T Nashed , Rodolfo Ghirlando , Victoria N Drago , Andrey Kovalevsky , John M Louis

Proline-rich domain of human ALIX contains multiple TSG101-UEV interaction sites and forms phosphorylation-mediated reversible amyloids.

Proceedings of the National Academy of Sciences of the United States of America

Avec: Ruben D Elias , Wen Ma , Rodolfo Ghirlando , Charles D Schwieters , Vijay S Reddy , Lalit Deshmukh

Distinct Structures and Dynamics of Chromatosomes with Different Human Linker Histone Isoforms.

Molecular cell

Avec: Bing-Rui Zhou , Hanqiao Feng , Seyit Kale , Tara Fox , Htet Khant , Natalia de Val , Rodolfo Ghirlando , Anna R Panchenko , Yawen Bai

Structure elucidation of the elusive Enzyme I monomer reveals the molecular mechanisms linking oligomerization and enzymatic activity.

Proceedings of the National Academy of Sciences of the United States of America

Avec: Trang T Nguyen , Rodolfo Ghirlando , Julien Roche , Vincenzo Venditti

The dimerization interface of initiator RctB governs chaperone and enhancer dependence of Vibrio cholerae chromosome 2 replication.

Nucleic acids research

Avec: Roopa Kothapalli , Rodolfo Ghirlando , Zaki Ali Khan , Soniya Chatterjee , Noemi Kedei , Dhruba K Chattoraj

Unmasking the Conformational Stability and Inhibitor Binding to SARS-CoV-2 Main Protease Active Site Mutants and Miniprecursor.

Journal of molecular biology

Avec: Andrey Kovalevsky , Leighton Coates , Daniel W Kneller , Rodolfo Ghirlando , Annie Aniana , Nashaat T Nashed , John M Louis

Phase Separation and Fibrillization of Human Annexin A7 Are Mediated by Its Proline-Rich Domain.

Biochemistry

Avec: Chenrong Yu , Spencer L Nelson , Georg Meisl , Rodolfo Ghirlando , Lalit Deshmukh

Biochemical and structural analyses reveal that the tumor suppressor neurofibromin (NF1) forms a high-affinity dimer.

The Journal of biological chemistry

Avec: Mukul Sherekar , Sae-Won Han , Rodolfo Ghirlando , Simon Messing , Matthew Drew , Dana Rabara , Timothy Waybright , Puneet Juneja , Hugh O'Neill , Christopher B Stanley , Debsindhu Bhowmik , Arvind Ramanathan , Sriram Subramaniam , Dwight V Nissley , William Gillette , Frank McCormick , Dominic Esposito

Application of millisecond time-resolved solid state NMR to the kinetics and mechanism of melittin self-assembly.

Proceedings of the National Academy of Sciences of the United States of America

Avec: Jaekyun Jeon , Kent R Thurber , Rodolfo Ghirlando , Wai-Ming Yau , Robert Tycko

Bacillus anthracis Virulence Regulator AtxA Binds Specifically to the

Journal of bacteriology

Avec: Rita M McCall , Mary E Sievers , Rasem Fattah , Rodolfo Ghirlando , Andrei P Pomerantsev , Stephen H Leppla

KRAS Prenylation Is Required for Bivalent Binding with Calmodulin in a Nucleotide-Independent Manner.

Biophysical journal

Avec: Constance Agamasu , Rodolfo Ghirlando , Troy Taylor , Simon Messing , Timothy H Tran , Lakshman Bindu , Marco Tonelli , Dwight V Nissley , Frank McCormick , Andrew G Stephen

Structures of ISCth4 transpososomes reveal the role of asymmetry in copy-out/paste-in DNA transposition.

The EMBO journal

Avec: Dalibor Kosek , Alison B Hickman , Rodolfo Ghirlando , Susu He , Fred Dyda

Transient lipid-bound states of spike protein heptad repeats provide insights into SARS-CoV-2 membrane fusion.

Science advances

Avec: Sai Chaitanya Chiliveri , John M Louis , Rodolfo Ghirlando , Ad Bax

HIV-1 matrix-tRNA complex structure reveals basis for host control of Gag localization.

Cell host & microbe

Avec: Charles Bou-Nader , Frauke Muecksch , Janae B Brown , Jackson M Gordon , Ashley York , Chen Peng , Rodolfo Ghirlando , Michael F Summers , Paul D Bieniasz , Jinwei Zhang

Cryo-EM structure of substrate-free

Current research in structural biology

Avec: Istvan Botos , George T Lountos , Weimin Wu , Scott Cherry , Rodolfo Ghirlando , Arsen M Kudzhaev , Tatyana V Rotanova , Natalia de Val , Joseph E Tropea , Alla Gustchina , Alexander Wlodawer

Bivalent recognition of fatty acyl-CoA by a human integral membrane palmitoyltransferase.

Proceedings of the National Academy of Sciences of the United States of America

Avec: Chul-Jin Lee , Robyn Stix , Mitra S Rana , Flowreen Shikwana , R Elliot Murphy , Rodolfo Ghirlando , José D Faraldo-Gómez , Anirban Banerjee

Autoprocessing and oxyanion loop reorganization upon GC373 and nirmatrelvir binding of monomeric SARS-CoV-2 main protease catalytic domain.

Communications biology

Avec: Nashaat T Nashed , Daniel W Kneller , Leighton Coates , Rodolfo Ghirlando , Annie Aniana , Andrey Kovalevsky , John M Louis

Réseau de co-auteurs

John M Louis 9 collaborations

Nashaat T Nashed 7 collaborations

Annie Aniana 7 collaborations

Andrey Kovalevsky 6 collaborations

Leighton Coates 5 collaborations

Lalit Deshmukh 5 collaborations

G Marius Clore 3 collaborations

Ruben D Elias 3 collaborations

Daniel W Kneller 3 collaborations

Alberto Ceccon 2 collaborations

Vitali Tugarinov 2 collaborations

Sai Chaitanya Chiliveri 2 collaborations

Alison B Hickman 2 collaborations

Fred Dyda 2 collaborations

Jinwei Zhang 2 collaborations

Charles D Schwieters 2 collaborations

Liang Zhang 2 collaborations

Jong Il Ahn 2 collaborations

Lingjun Meng 2 collaborations

Lixin Fan 2 collaborations

Yun-Xing Wang 2 collaborations

Jung-Eun Park 2 collaborations

Kyung S Lee 2 collaborations

Trang T Nguyen 2 collaborations

Vincenzo Venditti 2 collaborations

Victoria N Drago 2 collaborations

Min Li 2 collaborations

Stephen H Leppla 2 collaborations

Spencer L Nelson 2 collaborations

Natalia de Val 2 collaborations

Simon Messing 2 collaborations

Dwight V Nissley 2 collaborations

Frank McCormick 2 collaborations

Eric R Henry 1 collaboration

Troy Cellmer 1 collaboration

Emily B Dunkelberger 1 collaboration

Belhu Metaferia 1 collaboration

James Hofrichter 1 collaboration

Quan Li 1 collaboration

David Ostrowski 1 collaboration

Stéphane Moutereau 1 collaboration

Frédéric Galactéros 1 collaboration

Swee Lay Thein 1 collaboration

Pablo Bartolucci 1 collaboration

William A Eaton 1 collaboration

Wentian Luo 1 collaboration

Pavol Genzor 1 collaboration

Christopher M Furman 1 collaboration

Anna Menshikh 1 collaboration

Astrid Haase 1 collaboration

Matthew H Wilson 1 collaboration

Aline Umuhire Juru 1 collaboration

Samuel A Kotler 1 collaboration

Thomas Schmidt 1 collaboration

David S Libich 1 collaboration

Tae-Sung Kim 1 collaboration

Yang Chen 1 collaboration

Eunhye Lee 1 collaboration

Jeong Kyu Bang 1 collaboration

Jung Mi Lim 1 collaboration

Bo Yeon Kim 1 collaboration

Rochelle R Dotas 1 collaboration

Charles E Stewart 1 collaboration

Davit A Potoyan 1 collaboration

Harsha Ravishankar 1 collaboration

Klara Kirsch 1 collaboration

Yan Zeng 1 collaboration

Hye-Yeoung Yun 1 collaboration

Buyong Ma 1 collaboration

David Ball 1 collaboration

Bonsu Ku 1 collaboration

Ruth Nussinov 1 collaboration

Jeremy D Schmit 1 collaboration

William F Heinz 1 collaboration

Seung Jun Kim 1 collaboration

Tatiana Karpova 1 collaboration

Yingqi Zhu 1 collaboration

Qi Su 1 collaboration

Jin Zhang 1 collaboration

Lauren Wear 1 collaboration

Oksana Gerlits 1 collaboration

Mengli Cai 1 collaboration

Ying Huang 1 collaboration

Yang Shen 1 collaboration

Michiyo Mizuuchi 1 collaboration

Kiyoshi Mizuuchi 1 collaboration

Elyse S Fischer 1 collaboration

Warren A Campbell 1 collaboration

Shihui Liu 1 collaboration

Rasem J Fattah 1 collaboration

Thomas H Bugge 1 collaboration

Bhargavi Ramaraju 1 collaboration

Wenwei Zheng 1 collaboration

Aman Shihora 1 collaboration

John A Hammond 1 collaboration

Renbin Yang 1 collaboration

Xuemin Chen 1 collaboration

Huaibin Wang 1 collaboration

Emilios K Dimitriadis 1 collaboration

Robert Craigie 1 collaboration

Azamat Rizuan 1 collaboration

Jayakrishna Shenoy 1 collaboration

Priyesh Mohanty 1 collaboration

Patricia M S Dos Passos 1 collaboration

José F Mercado Ortiz 1 collaboration

Leanna Bai 1 collaboration

Renjith Viswanathan 1 collaboration

Szu-Huan Wang 1 collaboration

Victoria Johnson 1 collaboration

Lohany D Mamede 1 collaboration

Yuna M Ayala 1 collaboration

Jeetain Mittal 1 collaboration

Nicolas L Fawzi 1 collaboration

Wen Ma 1 collaboration

Vijay S Reddy 1 collaboration

Bing-Rui Zhou 1 collaboration

Hanqiao Feng 1 collaboration

Seyit Kale 1 collaboration

Tara Fox 1 collaboration

Htet Khant 1 collaboration

Anna R Panchenko 1 collaboration

Yawen Bai 1 collaboration

Julien Roche 1 collaboration

Roopa Kothapalli 1 collaboration

Zaki Ali Khan 1 collaboration

Soniya Chatterjee 1 collaboration

Noemi Kedei 1 collaboration

Dhruba K Chattoraj 1 collaboration

Chenrong Yu 1 collaboration

Georg Meisl 1 collaboration

Mukul Sherekar 1 collaboration

Sae-Won Han 1 collaboration

Matthew Drew 1 collaboration

Dana Rabara 1 collaboration

Timothy Waybright 1 collaboration

Puneet Juneja 1 collaboration

Hugh O'Neill 1 collaboration

Christopher B Stanley 1 collaboration

Debsindhu Bhowmik 1 collaboration

Arvind Ramanathan 1 collaboration

Sriram Subramaniam 1 collaboration

William Gillette 1 collaboration

Dominic Esposito 1 collaboration

Jaekyun Jeon 1 collaboration

Kent R Thurber 1 collaboration

Wai-Ming Yau 1 collaboration

Robert Tycko 1 collaboration

Rita M McCall 1 collaboration

Mary E Sievers 1 collaboration

Rasem Fattah 1 collaboration

Andrei P Pomerantsev 1 collaboration

Constance Agamasu 1 collaboration

Troy Taylor 1 collaboration

Timothy H Tran 1 collaboration

Lakshman Bindu 1 collaboration

Marco Tonelli 1 collaboration

Andrew G Stephen 1 collaboration

Dalibor Kosek 1 collaboration

Susu He 1 collaboration

Ad Bax 1 collaboration

Charles Bou-Nader 1 collaboration

Frauke Muecksch 1 collaboration

Janae B Brown 1 collaboration

Jackson M Gordon 1 collaboration

Ashley York 1 collaboration

Chen Peng 1 collaboration

Michael F Summers 1 collaboration

Paul D Bieniasz 1 collaboration

Istvan Botos 1 collaboration

George T Lountos 1 collaboration

Weimin Wu 1 collaboration

Scott Cherry 1 collaboration

Arsen M Kudzhaev 1 collaboration

Tatyana V Rotanova 1 collaboration

Joseph E Tropea 1 collaboration

Alla Gustchina 1 collaboration

Alexander Wlodawer 1 collaboration

Chul-Jin Lee 1 collaboration

Robyn Stix 1 collaboration

Mitra S Rana 1 collaboration

Flowreen Shikwana 1 collaboration

R Elliot Murphy 1 collaboration

José D Faraldo-Gómez 1 collaboration

Anirban Banerjee 1 collaboration