Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion.

Antiviral Agents / pharmacology COVID-19 / metabolism Endosomes / genetics Humans Hydroxycholesterols / pharmacology Interferons / metabolism Membrane Fusion / drug effects Severe acute respiratory syndrome-related coronavirus / drug effects SARS-CoV-2 / drug effects Spike Glycoprotein, Coronavirus / antagonists & inhibitors Virus Internalization / drug effects Virus Replication / drug effects COVID-19 Drug Treatment
COVID-19 SARS-CoV-2 innate immunity interferon virus entry

Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
15 12 2020
Historique:
pubmed: 27 11 2020
medline: 23 12 2020
entrez: 26 11 2020
Statut: ppublish

Résumé

Cholesterol 25-hydroxylase (

Identifiants

DOI: 10.1073/pnas.2012197117 PMID: 33239446 PMC: PMC7749331
pubmed: 33239446
pii: 2012197117
doi: 10.1073/pnas.2012197117
pmc: PMC7749331
doi:

Substances chimiques

Antiviral Agents 0
Hydroxycholesterols 0
Spike Glycoprotein, Coronavirus 0
spike protein, SARS-CoV-2 0
25-hydroxycholesterol 767JTD2N31
Interferons 9008-11-1

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

32105-32113

Subventions

Organisme : NIGMS NIH HHS
ID : R35 GM130386
Pays : United States
Organisme : NIAID NIH HHS
ID : R00 AI135031
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL107594
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007245
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI150796
Pays : United States
Organisme : NIAID NIH HHS
ID : R37 AI059371
Pays : United States
Organisme : BLRD VA
ID : I01 BX004235
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI127828
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK052574
Pays : United States
Organisme : NIH HHS
ID : GM130386
Pays : United States
Organisme : NIAID NIH HHS
ID : 75N93019C00062
Pays : United States

Informations de copyright

Copyright © 2020 the Author(s). Published by PNAS.

Déclaration de conflit d'intérêts

Competing interest statement: M.S.D. is a consultant for Inbios, Eli Lilly, Vir Biotechnology, NGM Biopharmaceuticals, and Emergent BioSolutions and on the Scientific Advisory Board of Moderna. The Diamond Laboratory at Washington University School of Medicine in St. Louis has received sponsored research agreements from Moderna. Invention disclosure filed with Washington University in St. Louis for the recombinant VSV-SARS-CoV-2 used herein. W.J.G. and Y.W. are listed as inventors on the patent “Kit and method for quantitative detection of steroids” US9851368B2. W.J.G., E.Y., and Y.W. are shareholders in CholesteniX Ltd.

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Auteurs

Ruochen Zang (R)

Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, 266100 Qingdao, China.
ORCID: 0000-0002-8496-7862

James Brett Case (JB)

Department of Medicine, Division of Infectious Diseases, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.

Eylan Yutuc (E)

Swansea University Medical School, SA2 8PP Swansea, United Kingdom.
ORCID: 0000-0001-9971-1950

Xiucui Ma (X)

Center for Cardiovascular Research and Division of Cardiology, Department of Internal Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO 63111.
John Cochran VA Medical Center, St. Louis, MO 63106.

Sheng Shen (S)

Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202.

Maria Florencia Gomez Castro (MF)

Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
ORCID: 0000-0003-4081-7819

Zhuoming Liu (Z)

Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
ORCID: 0000-0001-8198-0976

Qiru Zeng (Q)

Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.

Haiyan Zhao (H)

Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.

Juhee Son (J)

Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
Program in Molecular Cell Biology, Washington University School of Medicine, St. Louis, MO 63110.

Paul W Rothlauf (PW)

Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
Program in Virology, Harvard Medical School, Boston, MA 02115.
ORCID: 0000-0002-0941-4467

Alex J B Kreutzberger (AJB)

Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115.
Department of Cell Biology, Harvard Medical School, Boston, MA 02115.

Gaopeng Hou (G)

Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.

Hu Zhang (H)

Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202.
ORCID: 0000-0002-7447-8360

Sayantan Bose (S)

Autonomous Therapeutics, Inc., New York, NY 10013.
ORCID: 0000-0003-2397-4740

Xin Wang (X)

Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, 266100 Qingdao, China.
ORCID: 0000-0001-7467-6060

Michael D Vahey (MD)

Department of Biomedical Engineering, McKelvey School of Engineering, Washington University in St. Louis, St. Louis, MO 63110.

Kartik Mani (K)

Center for Cardiovascular Research and Division of Cardiology, Department of Internal Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO 63111.
John Cochran VA Medical Center, St. Louis, MO 63106.
ORCID: 0000-0002-4951-3478

William J Griffiths (WJ)

Swansea University Medical School, SA2 8PP Swansea, United Kingdom.
ORCID: 0000-0002-4129-6616

Tom Kirchhausen (T)

Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA 02115.
Department of Cell Biology, Harvard Medical School, Boston, MA 02115.
ORCID: 0000-0003-0559-893X

Daved H Fremont (DH)

Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
ORCID: 0000-0002-8544-2689

Haitao Guo (H)

Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202.
ORCID: 0000-0002-7146-916X

Abhinav Diwan (A)

Center for Cardiovascular Research and Division of Cardiology, Department of Internal Medicine, Washington University School of Medicine in St. Louis, St. Louis, MO 63111.
John Cochran VA Medical Center, St. Louis, MO 63106.
ORCID: 0000-0001-7554-4772

Yuqin Wang (Y)

Swansea University Medical School, SA2 8PP Swansea, United Kingdom.
ORCID: 0000-0002-3063-3066

Michael S Diamond (MS)

Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
Department of Medicine, Division of Infectious Diseases, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
Department of Pathology and Immunology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.

Sean P J Whelan (SPJ)

Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110.
ORCID: 0000-0003-1564-8590

Siyuan Ding (S)

Department of Molecular Microbiology, Washington University School of Medicine in St. Louis, St. Louis, MO 63110; siyuan.ding@wustl.edu.
ORCID: 0000-0002-5338-260X

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antiviraux › Cholesterol 25-hydroxylase suppresses...
questionsmedicales.fr › Maladies de l'appareil respiratoire › Infections de l'appareil respiratoire › Pneumopathie infectieuse › Pneumopathie virale › COVID-19 › Cholesterol 25-hydroxylase suppresses...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Vésicules cytoplasmiques › Vésicules de transport › Endosomes › Cholesterol 25-hydroxylase suppresses...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Cholesterol 25-hydroxylase suppresses...
questionsmedicales.fr › Lipides › Lipides membranaires › Stérols › Cholestérol › Oxystérols › Hydroxycholestérols › Cholesterol 25-hydroxylase suppresses...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Interférons › Cholesterol 25-hydroxylase suppresses...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Fusion membranaire › Cholesterol 25-hydroxylase suppresses...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Nidovirales › Coronaviridae › Coronavirus › Betacoronavirus › Virus du SRAS › Cholesterol 25-hydroxylase suppresses...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Nidovirales › Coronaviridae › Coronavirus › Betacoronavirus › Virus du SRAS › SARS-CoV-2 › Cholesterol 25-hydroxylase suppresses...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › Protéines de l'enveloppe virale › Protéines de fusion virale › Glycoprotéine de spicule des coronavirus › Cholesterol 25-hydroxylase suppresses...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Pénétration virale › Cholesterol 25-hydroxylase suppresses...
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Réplication virale › Cholesterol 25-hydroxylase suppresses...
questionsmedicales.fr › Thérapeutique › Traitement médicamenteux › Traitements médicamenteux de la COVID-19 › Cholesterol 25-hydroxylase suppresses...