A genetic variant of Burkholderia mallei detected in Kuwait: Consequences for the PCR diagnosis of glanders.
Burkholderia mallei
fliP
PCR diagnosis
glanders
insertion sequence
variant
Journal
Transboundary and emerging diseases
ISSN: 1865-1682
Titre abrégé: Transbound Emerg Dis
Pays: Germany
ID NLM: 101319538
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
revised:
02
08
2020
received:
07
05
2020
accepted:
04
08
2020
pubmed:
28
11
2020
medline:
1
7
2021
entrez:
27
11
2020
Statut:
ppublish
Résumé
Glanders is a contagious zoonotic disease caused by Burkholderia mallei. Following the detection of glanders positive horses using the OIE complement fixation test, the tissues of two horses were analysed by PCR. While PCR systems targeting the Burkholderia pseudomallei complex gave positive signals, the species-specific PCR systems targeting B. mallei (fliP-IS407A) and B. pseudomallei (orf11)-the OIE recommended targets-resulted in negative signals. However, the presence of B. mallei in these tissues was confirmed with a recently described B. mallei-specific real-time PCR system and genotyping with MLST- and SNP-based methods, performed on the most positive tissue, identified a genotype closely related to B. mallei strains recently isolated in the Middle East. This study leads to recommendations regarding the use of PCR systems for the molecular diagnosis of glanders, especially in regions where the circulating B. mallei strains have not yet been fully genetically characterized.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
960-963Subventions
Organisme : European Commission's Directorate-General for Health and Consumers
Informations de copyright
© 2020 Wiley-VCH GmbH.
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