Chlamydia pneumoniae and Mycoplasma pneumoniae in children with cystic fibrosis: impact on bacterial respiratory microbiota diversity.
Biodiversity
Child
Child, Preschool
Chlamydophila Infections
/ microbiology
Chlamydophila pneumoniae
/ genetics
Cystic Fibrosis
/ microbiology
DNA, Bacterial
Humans
Metagenomics
Microbiota
Mycoplasma pneumoniae
/ genetics
Pneumonia, Mycoplasma
/ microbiology
Prospective Studies
RNA, Ribosomal, 16S
Respiratory System
/ microbiology
Sputum
/ microbiology
Chlamydiales
cystic fibrosis
intracellular bacteria
metagenomics
pneumonia
pulmonary exacerbation
Journal
Pathogens and disease
ISSN: 2049-632X
Titre abrégé: Pathog Dis
Pays: United States
ID NLM: 101595366
Informations de publication
Date de publication:
06 01 2021
06 01 2021
Historique:
received:
03
07
2020
accepted:
26
11
2020
pubmed:
29
11
2020
medline:
5
10
2021
entrez:
28
11
2020
Statut:
ppublish
Résumé
The contribution of intracellular and fastidious bacteria in Cystic fibrosis (CF) pulmonary exacerbations, and progressive lung function decline remains unknown. This project aimed to explore their impact on bacterial microbiota diversity over time in CF children. Sixty-one children enrolled in the MUCOVIB multicentre prospective cohort provided 746 samples, mostly nasopharyngeal swabs, throat swabs and sputa which were analysed using culture, specific real-time qPCRs and 16S rRNA amplicon metagenomics. Chlamydia pneumoniae (n = 3) and Mycoplasma pneumoniae (n = 1) were prospectively documented in 6.6% of CF children. Microbiota alpha-diversity in children with a documented C. pneumoniae was highly variable, similarly to children infected with Staphylococcus aureus or Pseudomonas aeruginosa. The transition from routine follow-up visits to pulmonary exacerbation (n = 17) yielded variable changes in diversity indexes with some extreme loss of diversity. The high rate of C. pneumoniae detection supports the need for regular screenings in CF patients. A minor impact of C. pneumoniae on the microbial community structure was documented. Although detected in a single patient, M. pneumoniae should also be considered as a possible aetiology of lung infection in CF subjects.
Identifiants
pubmed: 33247928
pii: 6009034
doi: 10.1093/femspd/ftaa074
pmc: PMC7787906
pii:
doi:
Substances chimiques
DNA, Bacterial
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of FEMS.
Références
Clin Infect Dis. 2014 Apr;58(8):1198-9
pubmed: 24429432
J Bacteriol. 2018 Jan 24;200(4):
pubmed: 29084859
Nat Methods. 2016 Jul;13(7):581-3
pubmed: 27214047
PLoS One. 2013 Apr 22;8(4):e61217
pubmed: 23630581
Clin Chest Med. 2016 Mar;37(1):59-67
pubmed: 26857768
Appl Environ Microbiol. 2007 Aug;73(16):5261-7
pubmed: 17586664
Appl Environ Microbiol. 2000 Apr;66(4):1328-33
pubmed: 10742207
Acta Paediatr Scand. 1981 Sep;70(5):623-8
pubmed: 6798822
Diagn Microbiol Infect Dis. 2003 Feb;45(2):85-95
pubmed: 12614979
Clin Infect Dis. 1992 Jan;14(1):178-82
pubmed: 1571425
Clin Infect Dis. 2011 Oct;53(8):847-8
pubmed: 21921229
J Cyst Fibros. 2005 Mar;4(1):71-3
pubmed: 15752685
Int J Syst Evol Microbiol. 2017 May;67(5):1613-1617
pubmed: 28005526
Nat Genet. 1999 Apr;21(4):385-9
pubmed: 10192388
Expert Rev Anti Infect Ther. 2010 Jul;8(7):775-90
pubmed: 20586563
Future Microbiol. 2016;11(3):403-25
pubmed: 27028061
mBio. 2017 Sep 26;8(5):
pubmed: 28951476
Cochrane Database Syst Rev. 2012 Nov 14;11:CD002203
pubmed: 23152214
Infection. 2006 Dec;34(6):355-6
pubmed: 17180594
Proc Natl Acad Sci U S A. 2012 Apr 10;109(15):5809-14
pubmed: 22451929
Clin Infect Dis. 1996 May;22(5):819-23
pubmed: 8722938
Microbiome. 2015 Apr 01;3:12
pubmed: 25834733
Cell Host Microbe. 2011 Nov 17;10(5):497-506
pubmed: 22036469
Respir Res. 2017 May 19;18(1):98
pubmed: 28526018
J Pediatr. 2001 Sep;139(3):359-65
pubmed: 11562614
Acta Paediatr. 2020 Feb;109(2):375-380
pubmed: 31168877
Nat Methods. 2010 May;7(5):335-6
pubmed: 20383131
J Infect Dis. 1995 Jul;172(1):265-7
pubmed: 7797928
Front Microbiol. 2016 Apr 12;7:513
pubmed: 27148202