High Concentrations of Aspartame Induce Pro-Angiogenic Effects in Ovo and Cytotoxic Effects in HT-29 Human Colorectal Carcinoma Cells.
CAM assay
HT-29 cells
angiogenesis
aspartame
colorectal carcinoma
cytotoxicity
Journal
Nutrients
ISSN: 2072-6643
Titre abrégé: Nutrients
Pays: Switzerland
ID NLM: 101521595
Informations de publication
Date de publication:
24 Nov 2020
24 Nov 2020
Historique:
received:
31
10
2020
revised:
20
11
2020
accepted:
22
11
2020
entrez:
1
12
2020
pubmed:
2
12
2020
medline:
23
4
2021
Statut:
epublish
Résumé
Aspartame (ASP), an artificial sweetener abundantly consumed in recent years in an array of dietary products, has raised some concerns in terms of toxicity, and it was even suggested a link with the risk of carcinogenesis (colorectal cancer), though the present scientific data are rather inconclusive. This study aims at investigating the potential role of aspartame in colorectal cancer by suggesting two experimental approaches: (i) an in vitro cytotoxicity screening in HT-29 human colorectal carcinoma cells based on cell viability (Alamar blue assay), cell morphology and cell migration (scratch assay) assessment and (ii) an in ovo evaluation in terms of angiogenic and irritant potential by means of the chorioallantoic membrane method (CAM). The in vitro results showed a dose-dependent cytotoxic effect, with a significant decrease of viable cells at the highest concentrations tested (15, 30 and 50 mM) and morphological cellular changes. In ovo, aspartame (15 and 30 mM) proved to have a pro-angiogenic effect and a weak irritant potential at the vascular level. These data suggest new directions of research regarding aspartame's role in colorectal cancer.
Identifiants
pubmed: 33255204
pii: nu12123600
doi: 10.3390/nu12123600
pmc: PMC7760274
pii:
doi:
Substances chimiques
Angiogenesis Inducing Agents
0
Cytotoxins
0
Sweetening Agents
0
Aspartame
Z0H242BBR1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : The National Council for Financing Higher Education
ID : CNFIS-FDI-2019-0393
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