Bio-mechanical characterization of a CAD/CAM PMMA resin for digital removable prostheses.

Apoptosis Brillouin spectroscopy CAD/CAM Cytotoxicity Dynamic Mechanical Analysis PMMA SEM

Journal

Dental materials : official publication of the Academy of Dental Materials
ISSN: 1879-0097
Titre abrégé: Dent Mater
Pays: England
ID NLM: 8508040

Informations de publication

Date de publication:
03 2021
Historique:
received: 09 07 2020
revised: 15 10 2020
accepted: 06 11 2020
pubmed: 2 12 2020
medline: 24 4 2021
entrez: 1 12 2020
Statut: ppublish

Résumé

To compare the mechanical and biological features of a polymethylmethacrylate (PMMA) disc for CAD/CAM prostheses (test samples, TG) with a traditional resin (control samples, CG). Mechanical analysis was performed using Dynamic Mechanical Analysis (DMA) and Brillouin's micro-spectroscopy. Human keratinocyte morphology and adhesion were analyzed by scanning electronic microscopy (SEM), cytotoxicity by the MTT assay, apoptosis by flow cytometry and p53, p21 and bcl2 gene expression by real time PCR. TG exhibited a higher elastic modulus than CG (range 5100-5500 ± 114.3 MPa vs 3000-3300 ± 99.97 MPa). The Brillouin frequency was found at ω PMMA resins, as used in CAD/CAM technology, displayed suitable biocompatible and mechanical properties for removable prostheses.

Identifiants

pubmed: 33257084
pii: S0109-5641(20)30310-9
doi: 10.1016/j.dental.2020.11.003
pii:
doi:

Substances chimiques

Dental Implants 0
Polymethyl Methacrylate 9011-14-7

Types de publication

Journal Article

Langues

eng

Pagination

e118-e130

Informations de copyright

Copyright © 2020 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.

Auteurs

Stefano Pagano (S)

Department of Biomedical and Surgical Sciences, Odontostomatological University Centre: Chair Prof. Stefano Cianetti, University of Perugia, Perugia, Italy. Electronic address: stefano.pagano@unipg.it.

Guido Lombardo (G)

Department of Biomedical and Surgical Sciences, Odontostomatological University Centre: Chair Prof. Stefano Cianetti, University of Perugia, Perugia, Italy. Electronic address: guido.lombardo@unipg.it.

Silvia Caponi (S)

Istituto Officina dei Materiali del CNR (CNR-IOM), Perugia Unit, Department of Physics and Geology, University of Perugia, Perugia, Italy. Electronic address: silvia.caponi@cnr.it.

Egidia Costanzi (E)

Department of Experimental Medicine, Section of Biosciences and Medical Embryology, University of Perugia, Perugia, Italy. Electronic address: egidia.costanzi@unipg.it.

Alessandro Di Michele (A)

Department of Physics and Geology, University of Perugia, Perugia, Italy. Electronic address: alessandro.dimichele@collaboratori.unipg.it.

Stefano Bruscoli (S)

Department of Medicine, Section of Pharmacology, University of Perugia, Perugia, Italy. Electronic address: stefano.bruscoli@unipg.it.

Iva Xhimitiku (I)

Department of Engineering, University of Perugia, Perugia, Italy. Electronic address: ivaxhimyiku@gmail.com.

Maddalena Coniglio (M)

Department of Biomedical and Surgical Sciences, Odontostomatological University Centre: Chair Prof. Stefano Cianetti, University of Perugia, Perugia, Italy. Electronic address: conigliomaddalena@gmail.com.

Chiara Valenti (C)

Department of Biomedical and Surgical Sciences, Odontostomatological University Centre: Chair Prof. Stefano Cianetti, University of Perugia, Perugia, Italy. Electronic address: chiara.valenti@studenti.unipg.it.

Maurizio Mattarelli (M)

Department of Physics and Geology, University of Perugia, Perugia, Italy. Electronic address: maurizio.mattarelli@unipg.it.

Gianluca Rossi (G)

Department of Engineering, University of Perugia, Perugia, Italy. Electronic address: gianluca.rossi@unipg.it.

Stefano Cianetti (S)

Department of Biomedical and Surgical Sciences, Odontostomatological University Centre: Chair Prof. Stefano Cianetti, University of Perugia, Perugia, Italy. Electronic address: stefano.cianetti@unipg.it.

Lorella Marinucci (L)

Department of Experimental Medicine, Section of Biosciences and Medical Embryology, University of Perugia, Perugia, Italy. Electronic address: lorella.marinucci@unipg.it.

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Classifications MeSH