A Case-Study of the Molecular Diagnosis of Echinococcus multilocularis in Wild Boar with Comments on its Public Health Significance in Turkey.
Echinococcus multilocularis
PCR
Sequencing
Turkey
Wild Boar
Journal
The Journal of parasitology
ISSN: 1937-2345
Titre abrégé: J Parasitol
Pays: United States
ID NLM: 7803124
Informations de publication
Date de publication:
12 11 2020
12 11 2020
Historique:
entrez:
1
12
2020
pubmed:
2
12
2020
medline:
22
1
2021
Statut:
ppublish
Résumé
Echinococcus multilocularis is a parasite species of zoonotic importance which can be fatal to humans and causes Alveolar Echinococcosis (AE). This report describes the development of a cyst from the liver of a wild boar and molecular confirmation of its identification. The cyst material was obtained from the liver of a wild boar killed by hunters. Genomic DNA was extracted from the germinal layer of the cyst material, and 875 bp mitochondrial cytochrome c oxidase subunit I (COI) gene fragment was amplified by PCR and sequenced. A BLAST search matched 100% with published Echinococcus multilocularis sequences. This study confirms the occurrence of E. multilocularis in a wild boar for the first time in Turkey.
Identifiants
pubmed: 33260210
pii: 449011
doi: 10.1645/19-196
doi:
Substances chimiques
DNA, Helminth
0
Types de publication
Case Reports
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
730-734Informations de copyright
© American Society of Parasitologists 2020.