Binding of CCCTC-Binding Factor (CTCF) to the Minute Virus of Mice Genome Is Important for Proper Processing of Viral P4-Generated Pre-mRNAs.
Animals
CCCTC-Binding Factor
/ metabolism
DNA-Binding Proteins
/ metabolism
Fluorescent Antibody Technique
Gene Expression Regulation, Viral
Genome, Viral
Host-Pathogen Interactions
Humans
Introns
Mice
Minute Virus of Mice
/ physiology
Models, Biological
Mutation
Nucleoproteins
/ metabolism
Parvoviridae Infections
/ veterinary
Protein Binding
RNA Precursors
RNA, Messenger
RNA, Viral
Rodent Diseases
/ metabolism
Viral Proteins
/ metabolism
RNA processing
gene expression
minute virus of mice
parvovirus
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
30 11 2020
30 11 2020
Historique:
received:
23
10
2020
revised:
23
11
2020
accepted:
25
11
2020
entrez:
3
12
2020
pubmed:
4
12
2020
medline:
27
2
2021
Statut:
epublish
Résumé
Specific chromatin immunoprecipitation of salt-fractionated infected cell extracts has demonstrated that the CCCTC-binding factor (CTCF), a highly conserved, 11-zinc-finger DNA-binding protein with known roles in cellular and viral genome organization and gene expression, specifically binds the genome of Minute Virus of Mice (MVM). Mutations that diminish binding of CTCF to MVM affect processing of the P4-generated pre-mRNAs. These RNAs are spliced less efficiently to generate the R1 mRNA, and definition of the NS2-specific exon upstream of the small intron is reduced, leading to relatively less R2 and the generation of a novel exon-skipped product. These results suggest a model in which CTCF is required for proper engagement of the spliceosome at the MVM small intron and for the first steps of processing of the P4-generated pre-mRNA.
Identifiants
pubmed: 33266080
pii: v12121368
doi: 10.3390/v12121368
pmc: PMC7760686
pii:
doi:
Substances chimiques
CCCTC-Binding Factor
0
DNA-Binding Proteins
0
Nucleoproteins
0
RNA Precursors
0
RNA, Messenger
0
RNA, Viral
0
Viral Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : F32 AI131468
Pays : United States
Organisme : NIAID NIH HHS
ID : K99 AI148511
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI116595
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI046458
Pays : United States
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