Metabolic changes induced by oral glucose tests in horses and their diagnostic use.
EMS
biomarker
insulin dysregulation
metabolomics
oral glucose test
Journal
Journal of veterinary internal medicine
ISSN: 1939-1676
Titre abrégé: J Vet Intern Med
Pays: United States
ID NLM: 8708660
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
14
04
2020
revised:
16
11
2020
accepted:
20
11
2020
pubmed:
6
12
2020
medline:
29
6
2021
entrez:
5
12
2020
Statut:
ppublish
Résumé
Little is known about the implications of hyperinsulinemia on energy metabolism, and such knowledge might help understand the pathophysiology of insulin dysregulation. Describe differences in the metabolic response to an oral glucose test, depending on the magnitude of the insulin response. Twelve Icelandic horses in various metabolic states. Horses were subjected to 3 oral glucose tests (OGT; 0.5 g/kg body weight glucose). Basal, 120 and 180 minutes samples were analyzed using a combined liquid chromatography tandem mass spectrometry and flow injection analysis tandem mass spectrometry metabolomic assay. Insulin concentrations were measured using an ELISA. Analysis was performed using linear models and partial least-squares regression. The kynurenine : tryptophan ratio increased over time during the OGT (adjusted P-value = .001). A high insulin response was associated with lower arginine (adjusted P-value = .02) and carnitine (adjusted P-value = .03) concentrations. A predictive model using only baseline samples performed well with as few as 7 distinct metabolites (sensitivity, 86%; 95% confidence interval [CI], 81%-90%; specificity, 88%; 95% CI, 84%-92%). Our results suggest induction of low-grade inflammation during the OGT. Plasma arginine and carnitine concentrations were lower in horses with high insulin response and could constitute potential therapeutic targets. Development of screening tools to identify insulin-dysregulated horses using only baseline blood sample appears promising.
Sections du résumé
BACKGROUND
BACKGROUND
Little is known about the implications of hyperinsulinemia on energy metabolism, and such knowledge might help understand the pathophysiology of insulin dysregulation.
OBJECTIVES
OBJECTIVE
Describe differences in the metabolic response to an oral glucose test, depending on the magnitude of the insulin response.
ANIMALS
METHODS
Twelve Icelandic horses in various metabolic states.
METHODS
METHODS
Horses were subjected to 3 oral glucose tests (OGT; 0.5 g/kg body weight glucose). Basal, 120 and 180 minutes samples were analyzed using a combined liquid chromatography tandem mass spectrometry and flow injection analysis tandem mass spectrometry metabolomic assay. Insulin concentrations were measured using an ELISA. Analysis was performed using linear models and partial least-squares regression.
RESULTS
RESULTS
The kynurenine : tryptophan ratio increased over time during the OGT (adjusted P-value = .001). A high insulin response was associated with lower arginine (adjusted P-value = .02) and carnitine (adjusted P-value = .03) concentrations. A predictive model using only baseline samples performed well with as few as 7 distinct metabolites (sensitivity, 86%; 95% confidence interval [CI], 81%-90%; specificity, 88%; 95% CI, 84%-92%).
CONCLUSIONS AND CLINICAL IMPORTANCE
CONCLUSIONS
Our results suggest induction of low-grade inflammation during the OGT. Plasma arginine and carnitine concentrations were lower in horses with high insulin response and could constitute potential therapeutic targets. Development of screening tools to identify insulin-dysregulated horses using only baseline blood sample appears promising.
Identifiants
pubmed: 33277752
doi: 10.1111/jvim.15992
pmc: PMC7848347
doi:
Substances chimiques
Blood Glucose
0
Insulin
0
Glucose
IY9XDZ35W2
Carnitine
S7UI8SM58A
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
597-605Informations de copyright
© 2020 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC. on behalf of the American College of Veterinary Internal Medicine.
Références
Vet Immunol Immunopathol. 2012 Oct 15;149(3-4):208-15
pubmed: 22871576
J Proteome Res. 2012 Feb 3;11(2):1373-81
pubmed: 22098372
Equine Vet J. 2014 Jan;46(1):103-12
pubmed: 24033478
Curr Opin Cell Biol. 2007 Aug;19(4):426-35
pubmed: 17651957
Diabetes. 2013 Aug;62(8):2689-98
pubmed: 23382451
Mol Syst Biol. 2008;4:214
pubmed: 18682704
J Biomed Biotechnol. 2012;2012:805683
pubmed: 22665992
Vet Immunol Immunopathol. 2009 Jun 15;129(3-4):216-20
pubmed: 19108899
Nucleic Acids Res. 2015 Apr 20;43(7):e47
pubmed: 25605792
Endocrinology. 1988 Sep;123(3):1598-604
pubmed: 2456916
J Vet Intern Med. 2021 Jan;35(1):597-605
pubmed: 33277752
J Vet Intern Med. 2019 Mar;33(2):335-349
pubmed: 30724412
Vet Rec. 2015 Aug 15;177(7):173-9
pubmed: 26273009
Domest Anim Endocrinol. 2018 Apr;63:1-9
pubmed: 29172109
Comp Biochem Physiol A Mol Integr Physiol. 2001 Jun;129(2-3):563-75
pubmed: 11423326
J Clin Endocrinol Metab. 1971 Nov;33(5):829-37
pubmed: 5125386
Trends Biotechnol. 2017 Aug;35(8):728-742
pubmed: 28456344
Physiol Rev. 2018 Oct 1;98(4):2133-2223
pubmed: 30067154
Pancreas. 1991 Sep;6(5):522-7
pubmed: 1946308
J Lipid Res. 2014 Apr;55(4):635-44
pubmed: 24395925
PLoS One. 2016 Sep 29;11(9):e0163815
pubmed: 27684374
J Anim Physiol Anim Nutr (Berl). 2009 Apr;93(2):203-8
pubmed: 19320933
Equine Vet J. 2017 Sep;49(5):570-576
pubmed: 28543410
Trends Mol Med. 2013 Mar;19(3):176-86
pubmed: 23375873
Can J Vet Res. 2015 Oct;79(4):329-38
pubmed: 26424915
J Vet Intern Med. 2010 May-Jun;24(3):467-75
pubmed: 20384947
J Vet Intern Med. 2019 Jan;33(1):225-232
pubmed: 30506731
J Chronic Dis. 1974 Dec;27(11-12):573-9
pubmed: 4436423
Ann Med. 2016;48(1-2):89-102
pubmed: 26883715
Obesity (Silver Spring). 2014 Jan;22(1):195-201
pubmed: 23625535
J Vet Intern Med. 2013 May-Jun;27(3):576-82
pubmed: 23517373
Aust Vet J. 2014 Apr;92(4):101-6
pubmed: 24673135
Vet J. 2007 Nov;174(3):530-5
pubmed: 17719811
Vet Immunol Immunopathol. 2012 Jan 15;145(1-2):283-9
pubmed: 22169327
J Clin Endocrinol Metab. 1971 Sep;33(3):409-17
pubmed: 4936881
Amino Acids. 2010 Jul;39(2):349-57
pubmed: 20437186
Equine Vet J. 2010 Mar;42(2):129-35
pubmed: 20156248
Arterioscler Thromb Vasc Biol. 2000 Sep;20(9):2032-7
pubmed: 10978245
BMC Vet Res. 2018 May 2;14(1):146
pubmed: 29716602
J Vet Intern Med. 2018 May;32(3):1215-1233
pubmed: 29572947
Bioinformatics. 2003 Jan 22;19(2):185-93
pubmed: 12538238
Metabolites. 2020 Apr 23;10(4):
pubmed: 32340170
J Anim Sci. 2007 May;85(5):1144-55
pubmed: 17264235
Vet Immunol Immunopathol. 2011 Aug 15;142(3-4):141-6
pubmed: 21621276