Identification of large genetic variations in the equine infectious anemia virus tat-gag genomic region.
Pantanal
equine infectious anaemia virus
horse
phylogenetic analysis
Journal
Transboundary and emerging diseases
ISSN: 1865-1682
Titre abrégé: Transbound Emerg Dis
Pays: Germany
ID NLM: 101319538
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
revised:
15
09
2020
received:
01
07
2020
accepted:
01
12
2020
pubmed:
8
12
2020
medline:
26
11
2021
entrez:
7
12
2020
Statut:
ppublish
Résumé
The aetiological agent of equine infectious anaemia (EIA) is the retrovirus equine infectious anemia virus (EIAV) that infects all members of the Equidae family. The EIA is widely disseminated in the Brazilian territory with a high seroprevalence in the Brazilian Pantanal and is mainly diagnosed using agar gel immunodiffusion (AGID). There are few complete EIAV genome sequences available in GenBank, which had an impact on molecular detection studies. In this study, we conducted molecular detection and sequencing of EIAV proviral DNA from Brazilian horses. We analysed the genomic region from exon 1 of tat to gag (tat-gag). Comparative serological tests, comprising AGID and two enzyme-linked immunosorbent assays (ELISAs), were also conducted. Of the 133 samples, 58 were positive in the tat-gag PCR, and 49 nucleotide sequences of 272 bp were obtained. Using this developed tat-gag PCR EIAV proviral DNA was detected in 7% of the AGID-negative samples and 26% of the AGID-negative samples were positive in at least one of the ELISA tests used. Using phylogenetic analysis, the Brazilian Pantanal EIAV sequences grouped in a different clade of EIAV sequences from other countries. Thus, the EIAV sequences can contribute to the knowledge of the tat-gag genomic region in the circulating viruses in the Brazilian Pantanal, in addition to providing new information about the genetic diversity. In addition, the serological results demonstrate the greater sensitivity of the ELISAs used in this study compared to AGID for EIA diagnosis.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3424-3432Subventions
Organisme : Empresa Brasileira de Pesquisa Agropecuária
ID : 02.12.01.030.00.00
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 307423/2015-8
Organisme : Fundação de Amparo à Pesquisa do Estado de Minas Gerais
Organisme : Fundação de Amparo à Pesquisa do Estado de Mato Grosso
ID : 330980/2012
Informations de copyright
© 2020 Wiley-VCH GmbH.
Références
Almeida, V. M. A., Gonçalves, V. S. P., Martins, M. F., Haddad, J. P. A., Dias, R. A., Leite, R. C., & Reis, J. K. P. (2006). Equine infectious anemia: Prevalence in working equids of livestock herds, in Minas Gerais, Brazil. Arquivo Brasileiro De Medicina Veterinária E Zootecnia, 58(2), 141-148. https://doi.org/10.1590/S0102-09352006000200001
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403-410. https://doi.org/10.1016/S0022-2836(05)80360-2
Barros, A. T. M. (2001). Seasonality and relative abundance of Tabanidae (Diptera) captured on horses in the Pantanl, Brazil. Memórias do Instituto Oswaldo Cruz, Rio De Janeiro, 96, 917-923. https://doi.org/10.1590/S0074-02762001000700006
Barros, M. L., Borges, A. M. C., De Oliveira, A. C. S., Lacerda, W., De Souza, A., & Aguiar, D. M. (2018). Spatial distribution and risk factors for equine infectious anaemia in the state of Mato Gross, Brazil. Revue Scientifique Et Technique, 37(3), 971-983. https://doi.org/10.20506/37.3.2900
Bazzo, J. C., De Freitas, D. A. F., Silva, M. L. N., Cardoso, E. L., & Santos, S. A. (2012). Aspectos Geofísicos e Ambientais do Pantanal da Nhecolândia. Revista De Geografia (UFPE), 29(1), 141-161.
Borges, A. M. C. M., Silva, L. G., Nogueira, M. F., Oliveira, A. C. S., Segri, N. J., Ferreira, F., Witter, R., & Aguiar, D. M. (2013). Prevalence and risk factors for Equine Infectious Anemia in Poconé municipality, northern Brazilian Pantanal. Research in Veterinary Science, 95(1), 76-81. https://doi.org/10.1016/j.rvsc.2013.02.011
Caij, A. B., & Tignon, M. (2014). Epidemiology and genetic characterization of equine infectious anaemia virus strains isolated in Belgium in 2010. Transboundary and Emerging Diseases, 61(5), 464-468. https://doi.org/10.1111/tbed.12031
Capomaccio, S., Cappelli, K., Cook, R. F., Nardi, F., Gifford, R., Marenzoni, M. L., & Passamonti, F. (2012). Geographic structuring of global EIAV isolates: A single origin for New World strains? Virus Research, 163(2), 656-659. https://doi.org/10.1016/j.virusres.2011.11.011
Capomaccio, S., Willand, Z. A., Cook, S. J., Issel, C. J., Santos, E. M., Reis, J. K. P., & Cook, R. F. (2012). Detection, molecular characterization and phylogenetic analysis of full-length equine infectious anemia (EIAV) gag genes isolated from Shackleford Banks wild horses. Veterinary Microbiology, 157(3-4), 320-332. https://doi.org/10.1016/j.vetmic.2012.01.015
Cappelli, K., Capomaccio, S., Cook, F. R., Felicetti, M., Marenzoni, M. L., Coppola, G., Verini-Supplizi, A., Coletti, M., & Passamonti, F. (2011). Molecular detection, epidemiology and genetic characterization of novel European field isolates of equine infectious anemia virus. Journal of Clinical Microbiology, 49(1), 27-33. https://doi.org/10.1128/JCM.01311-10
Cappelli, K., Cook, R. F., Stefanetti, V., Passamonti, F., Autorino, G. L., Scicluna, M. T., Coletti, M., Verini, S. A., & Capomaccio, S. (2017). Deep sequencing and variant analysis of an Italian pathogenic field strain of equine infectious anaemia virus. Transboundary and Emerging Diseases, 64(6), 2104-2112. https://doi.org/10.1111/tbed.12631
Cook, R. F., Leroux, C., Cook, S. J., Berger, S. L., Lichtenstein, D. L., Ghabrial, N. N., Montelaro, R. C., & Issel, C. J. (1998). Development and characterization of an in vivo pathogenic molecular clone of equine infectious anemia virus. Journal of Virology, 72(2), 1383-1393. https://doi.org/10.1128/JVI.72.2.1383-1393.1998
Cook, R. F., Leroux, C., & Issel, C. J. (2013). Equine infectious anemia and equine infectious anemia virus in 2013: A review. Veterinary Microbiology, 167(1-2), 181-204. https://doi.org/10.1016/j.vetmic.2013.09.031
Craigo, J. K., & Montelaro, R. C. (2013). Lessons in AIDS vaccine development learned from studies of equine infectious, anemia virus infection and immunity. Viruses, 5(12), 2963-2976. https://doi.org/10.3390/v5122963
Cullinane, A., Quinlivan, M., Nelly, M., Patterson, H., Kenna, R., Garvey, M., … Jankowska, K. (2007). Diagnosis of equine infectious anaemia during the 2006 outbreak in Ireland. Veterinary Record, 161(19), 647-652. https://doi.org/10.1136/vr.161.19.647
Cursino, A. E., Vilela, A. P. P., Franco-Luiz, A. P. M., de Oliveira, J. G., Nogueira, M. F., Júnior, J. P. A., de Aguiar, D. M., & Kroon, E. G. (2018). Equine infectious anemia virus in naturally infected horses from the Brazilian Pantanal. Archives of Virology, 163(9), 2385-2394. https://doi.org/10.1007/s00705-018-3877-8
Da Silva, J. S. V., & Abdon, M. M. (1998). Delimitação do Pantanal brasileiro e suas sub-regiões. Pesquisa Agropecuária Brasileira, Brasília, 33, 1703-1711.
Darriba, D., Taboada, G. L., Doallo, R., & Posada, D. (2012). jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772. https://doi.org/10.1038/nmeth.2109
Domingo, E., Sheldon, J., & Perales, C. (2012). Viral quaispecies evolution. Microbiology and Molecular Biology Reviews, 76(2), 159-216. https://doi.org/10.1128/MMBR.05023-11
Dong, J. B., Zhu, W., Cook, F. R., Goto, Y., Horii, Y., & Haga, T. (2013). Identification of a novel equine infectious anemia virus field strain isolated from feral horses in southern Japan. Journal of General Virology, 94(2), 360-365. https://doi.org/10.1099/vir.0.047498-0
Gaudaire, D., Lecouturier, F., Ponçon, N., Morilland, E., Laugier, C., Zientara, S., & Hans, A. (2018). Molecular characterization of equine infectious anemia virus from a major outbreak in southeastern France. Transboudary and Emerging Diseases, 65(1), 7-13. https://doi.org/10.1111/tbed.12657
Goff, S. P. (2013). Retroviridae. In D. M. Knipe, & P. M. Howley (Eds.), Fields Virology (pp. 1424-1473). 6th Edition, Volume 2, chapter 47.
Hurt, C. B., Nelson, J. A. E., Hightow-Weidman, L. B., & Miller, W. C. (2017). Selecting an HIV test: A narrative review for clinical and researchers. Sexually Transmitted Diseases, 44(12), 739-746. https://doi.org/10.1097/OLQ.0000000000000719
International Committee on Taxonomy of Viruses (ICTV). (2018). Retroviridae. Retrieved from https://talk.ictvonline.org/
Issel, C. J., Cook, R. F., Mealey, R. H., & Horohov, D. W. (2014). Equine infectious anemia in 2014: Live with it or eradicate it? Veterinary Clinics of North America: Equine Practice, 30(3), 561-577. https://doi.org/10.1016/j.cveq.2014.08.002
Issel, C. J., Rushlow, K., Foil, L. D., & Montelaro, R. C. (1998). A perspective on equine infectious anemia with an emphasis on vector transmission and genetic analysis. Veterinary Microbiology, 17(3), 251-286. https://doi.org/10.1016/0378-1135(88)90069-7
Junk, W. J., Da Cunha, C. N., Wantzen, K. M., Petermann, P., Strüssmann, C., Marques, M. I., & Adis, J. (2006). Biodiversity and its conservation in the Pantanal of Mato Grosso, Brazil. Aquatic Sciences, 68, 278-309. https://doi.org/10.1007/s00027-006-0851-4
Kawakami, T., Sherman, L., Dahlberg, J., Gazit, A., Yaniv, A., Tronick, S. R., & Aaronson, S. A. (1987). Nucleotide sequence analysis of equine infectious anemia virus proviral DNA. Virology, 158(2), 300-312. https://doi.org/10.1016/0042-6822(87)90202-9
Kuhar, U., & Malovrh, T. (2016). High genetic diversity of equine infectious anaemia virus strains from Slovenia revealed upon phylogenetic analysis of the p15 gag gene region. Equine Veterinary Journal, 48(2), 205-210. https://doi.org/10.1111/evj.12404
Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547-1549. https://doi.org/10.1093/molbev/msy096
Leroux, C., Cadoré, J. L., & Montelaro, R. C. (2004). Equine infectious anemia virus (EIAV): What has HIV’s country cousin go to tell us? Veterinary Research, 35(4), 485-512. https://doi.org/10.1051/vetres:2004020
Malossi, C. D., Fioratti, E. G., Cardoso, J. F., Magro, A. J., Kroon, E. G., De Aguiar, D. M., Borges, A. M. C. M., Nogueira, M. F., Ullmann, L. S., & Araújo Júnior, J. P. (2020). High genomic variability in equine infectious anemia virus obtained from naturally infected horses in Pantanal, Brazil: An endemic region case. Viruses, 12(2), 207. https://doi.org/10.3390/v12020207
MAPA-a Ministério da Agricultura Pecuária e Abastecimento (MAPA)/ Secretaria de Defesa Agropecuária. Instrução Normativa Nº 52, de 26 de novembro de. (2018). Retrieved from http://www.in.gov.br/materia/-/asset_publisher/Kujrw0TZC2Mb/content/id/52002092/do1-2018-11-27-instrucao-normativa-n-52-de-26-de-novembr. Accessed September 09 2019
MAPA-b Ministério da Agricultura, Pecuária e Abastecimento (MAPA). Instrução Normativa Nº 45, de 15 de Junho de. (2004). Retrieved from http://www.cidasc.sc.gov.br/defesasanitariaanimal/files/2012/10/Instrução-normativa-n°-45-Aprova-as-Normas-para-a-Prevenção-e-o-Controle-da-Anemia-Infecciosa-Eqüina-A.I.E.1.pdf. Accessed September 09 2019
McGuire, T. C., Leib, S. R., Lonning, S. M., Zhang, W., Byrne, K. M., & Mealey, R. H. (2000). Equine infectious anaemia virus proteins with epitopes most frequently recognized by cytotoxic T lymphocytes from infected horses. Journal of General Virology, 81(11), 2735-2739. https://doi.org/10.1099/0022-1317-81-11-2735
Nagarajan, M. M., & Simard, C. (2007). Gag genetic heterogeneity of equine infectious anemia virus (EIAV) in naturally infected horses in Canada. Virus Research, 129(2), 228-235. https://doi.org/10.1016/j.virusres.2007.07.013
Nogueira, M. F., Oliveira, J. M., Santos, C. J. S., Petzold, H. V., Aguiar, D. M., Juliano, R. S., Reis, J. K. P., & Abreu, U. G. P. (2017). Equine infectious anaemia in equids of Southern Pantanal, Brazil: Seroprevalence and evaluation of the adoption of a control programme. Pesquisa Veterinária Brasileira, 37, 227-233. https://doi.org/10.1590/s0100-736x2017000300005
OIE-a Listed diseases, infections and infestations in force in. (2019). World Organisation For Animal Health. Retrieved from http://www.oie.int/animal-health-in-the-world/oie-listed-diseases-2019. Accessed 09 September 2019
OIE-b Terrestrial manual. (2019). Chapter 3.5.6 Equine infectious anaemia. World Organisation For Animal Health (OIE). Retrieved from https://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/3.05.06_EIA.pdf. Accessed 10 september 2019
Petropoulos, C. (1997). Retroviral taxonomy, protein structures, sequences, and genetic maps. In J. M. Coffin, S. H. Hughes, & H. E. Varmus (Eds.), Retroviruses. Cold Spring Harbor Laboratory Press.
Quinlivan, M., Cook, F., Kenna, R., Callinan, J. J., & Cullinane, A. (2013). Genetic characterization by composite sequence analysis of a new pathogenic field strain of equine infectious anemia virus from the 2006 outbreak in Ireland. Journal General of. Virology, 94(3), 612-622. https://doi.org/10.1099/vir.0.047191-0
Sanger, F., Nicklen, S., & Coulson, A. R. (1997). DNA sequencing with chain-terminating inhibitors. PNAS, 74(12), 5463-5467. https://doi.org/10.1073/pnas.74.12.5463
Santos, A. S., Salis, S. M., Comastri, F. J. A. (2016). Cavalo Pantaneiro: Rústico por natureza (p. 603p). Embrapa Pantanal.
Sherman, L., Gazit, A., Yaniv, A., Kawakami, T., Dahlberg, J. E., & Tronick, S. R. (1988). Localization of sequences responsible for trans-activation of the equine infectious anemia virus long terminal repeat. Journal of Virology, 62(1), 120-126. https://doi.org/10.1128/JVI.62.1.120-126.1988
Silva, R. A. M. S., Abreu, U. G. P., & Barros, A. T. M. (2001). Anemia Infecciosa Eqüina: Epizootiologia, Prevenção e Controle no Pantanal. Corumbá: Embrapa Pantanal (Embrapa Pantanal Circular Técnica, 29). Retrieved from https://www.infoteca.cnptia.embrapa.br/bitstream/doc/807376/1/CT29.pdf. Accessed 09 September 2019
Silva, R. A. M. S., Barros, A. T. M., CostaNeto, A. A., Lopes, N., Cortada, V. M. C., Matsuura, T. M.,de, S. M., … Bandini, O. (2004). Programa de prevenção e controle da anemia infecciosa equina no Pantanal sul-mato-grossense. Corumbá, Embrapa Pantanal. Retrieved from https://www.embrapa.br/busca-de-publicacoes/-/publicacao/812061/programa-de-prevencao-e-controle-da-anemia-infecciosa-equina-no-pantanal-sul-mato-grossense. Accessed 09 September 2019
Souza, G. S., Gomes, E. G., Santos, S. A., De Rezende, A. S. C., Andrade, D. R. F., Nogueira, M. F., Trigo, P., & De Abreu, U. G. P. (2018). Factors affecting the performance of Pantaneiro horses. Revista Brasileira De Zootecnia, 47, https://doi.org/10.1590/rbz4720170162
Stucky, B. J. (2012). SeqTrace: A graphical tool for rapidly processing DNA sequencing chromatograms. Journal of Biomolecular Techniques, 23(3), 90-93. https://doi.org/10.7171/jbt.12-2303-004
Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12), 2725-2729. https://doi.org/10.1093/molbev/mst197
Tigre, D. M., Brandão, C. F. L., De Paula, F. L., Chinalia, F. A., Campos, G. S., & Sardi, S. I. (2017). Characterization of isolates of equine infectious anemia virus in Brazil. Archives of Virology, 162(3), 873-877. https://doi.org/10.1007/s00705-016-3172-5
Tu, Y. B., Zhou, T., Yuan, X. F., Qiu, H. J., Xue, F., Sun, C. Q., … Tong, G. Z. (2007). Long terminal repeats are not the sole determinants of virulence for equine infectious anemia virus. Archives of Virology, 152, 209-218. https://doi.org/10.1007/s00705-006-0830-z
Wang, X., Wang, S., Lin, Y., Jiang, C., Ma, J., Zhao, L., Lv, X., Wang, F., Shen, R., Kong, X., & Zhou, J. (2011). Genomic comparison between attenuated Chinese equine infectious anemia virus vaccine strains and their parental virulent strains. Archives of Virology, 156(2), 353-357. https://doi.org/10.1007/s00705-010-0877-8
Zheng, Y. H., Sentsui, H., Kono, Y., & Ikuta, K. (2000). Mutations occurring during serial passage of Japanese equine infectious anemia virus in primary horse macrophages. Virus Research, 68(1), 93-98. https://doi.org/10.1016/S0168-1702(00)00147-7