p53 Gene Mutation in Blood of Zebu Cattle with Squamous Cell Carcinoma of the Horn.
cattle
horn cancer
mutation
p53 gene
Journal
Journal of comparative pathology
ISSN: 1532-3129
Titre abrégé: J Comp Pathol
Pays: England
ID NLM: 0102444
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
02
05
2020
revised:
29
06
2020
accepted:
29
09
2020
entrez:
8
12
2020
pubmed:
9
12
2020
medline:
9
11
2021
Statut:
ppublish
Résumé
Horn cancer is one of the most important diseases in Zebu castrated male cattle. The objective of this study was to investigate the presence of p53 gene mutation in the blood of affected cattle and its value for early diagnosis and prognosis. The study was conducted on blood samples from 20 affected cattle and six healthy control cattle from Western India. Plasma samples were evaluated for the presence of p53 gene mutation using the polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) technique and the results were correlated with the stage of cancer. Five of the 20 cases had stage I neoplasms, nine stage II and six stage III, based on histopathological examination. PCR-SSCP analysis revealed an aberrant pattern of DNA migration on polyacrylamide gel electrophoresis of DNA extracts from blood samples of six animals with stage II and stage III cancer. No mutation was identified in blood from cattle with stage I cancer or from healthy control cattle. These results suggest that PCR-SSCP detection of p53 gene mutation in blood has potential diagnostic and prognostic value, and indicate the need for further large-scale investigation.
Identifiants
pubmed: 33288151
pii: S0021-9975(20)30127-4
doi: 10.1016/j.jcpa.2020.09.018
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
53-57Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.