Hypoxia and CD11b+ Cell Influx Are Strongly Associated With Lymph Node Metastasis of Oral Cancer.
Animals
CD11b Antigen
/ metabolism
Cell Line, Tumor
Cell Movement
Disease Models, Animal
Fluorescent Antibody Technique
Humans
Hypoxia
/ metabolism
Lymphangiogenesis
Lymphatic Metastasis
Lymphocytes
/ metabolism
Mice
Mouth Neoplasms
/ metabolism
Neoplasm Staging
Neovascularization, Pathologic
/ metabolism
Xenograft Model Antitumor Assays
CD11b+ cell
LN metastasis
Oral cancer
hypoxia
lymphangiogenesis
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
06
11
2020
revised:
14
11
2020
accepted:
17
11
2020
entrez:
8
12
2020
pubmed:
9
12
2020
medline:
22
12
2020
Statut:
ppublish
Résumé
Treatment failure in oral cancer is mainly caused by uncontrolled cervical lymph node (LN) metastasis. We previously reported that CD11b+ cells are recruited into tumor hypoxic areas following radiation, leading to re-vascularization and relapse. Since lymphatic vessel formation has similarities with vascular formation, we examined whether surgery induces hypoxia and stimulates lymphangiogenesis. The recruitment of CD11b+ cells and the formation of lymphatic vessels were examined using orthotopic tongue cancer mouse models with glossectomy. Surgery on OSC-19 tumor induced LN metastases and hypoxia, followed by CD11b+ cell influx. These phenomena were not observed in the no tumor or SAT tumor models. Stimulation of lymphangiogenesis was observed in the CD11b+ cell influx area, as the tumor grew. The localization of CD11b+ cells was changed from the lymph nodules to the medullary sinuses. Surgery-induced hypoxia in oral tumors leads to CD11b+ cell infiltration, lymphangiogenesis, and LN metastasis.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
Treatment failure in oral cancer is mainly caused by uncontrolled cervical lymph node (LN) metastasis. We previously reported that CD11b+ cells are recruited into tumor hypoxic areas following radiation, leading to re-vascularization and relapse. Since lymphatic vessel formation has similarities with vascular formation, we examined whether surgery induces hypoxia and stimulates lymphangiogenesis.
MATERIALS AND METHODS
METHODS
The recruitment of CD11b+ cells and the formation of lymphatic vessels were examined using orthotopic tongue cancer mouse models with glossectomy.
RESULTS
RESULTS
Surgery on OSC-19 tumor induced LN metastases and hypoxia, followed by CD11b+ cell influx. These phenomena were not observed in the no tumor or SAT tumor models. Stimulation of lymphangiogenesis was observed in the CD11b+ cell influx area, as the tumor grew. The localization of CD11b+ cells was changed from the lymph nodules to the medullary sinuses.
CONCLUSION
CONCLUSIONS
Surgery-induced hypoxia in oral tumors leads to CD11b+ cell infiltration, lymphangiogenesis, and LN metastasis.
Identifiants
pubmed: 33288576
pii: 40/12/6845
doi: 10.21873/anticanres.14706
doi:
Substances chimiques
CD11b Antigen
0
ITGAM protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6845-6852Informations de copyright
Copyright © 2020 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.