Dynamics of Chemical Diversity during Co-Cultures: An Integrative Time-Scale Metabolomics Study of Fungal Endophytes Cophinforma mamane and Fusarium solani.
Cophinforma mamane
Fusarium solani
co-cultures
metabolomics
time-scale analysis
Journal
Chemistry & biodiversity
ISSN: 1612-1880
Titre abrégé: Chem Biodivers
Pays: Switzerland
ID NLM: 101197449
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
13
08
2020
accepted:
07
12
2020
pubmed:
9
12
2020
medline:
3
7
2021
entrez:
8
12
2020
Statut:
ppublish
Résumé
A rapid and efficient metabolomic study of Cophinforma mamane and Fusarium solani co-cultivation in time-series based analysis was developed to study metabolome variations during their fungal interactions. The fungal metabolomes were studied through the integration of four metabolomic tools: MS-DIAL, a chromatographic deconvolution of liquid-chromatography-mass spectrometry (LC/MS); MS-FINDER, a structure-elucidation program with a wide range metabolome database; GNPS, an effective method to organize MS/MS fragmentation spectra, and MetaboAnalyst, a comprehensive web application for metabolomic data analysis and interpretation. Co-cultures of C. mamane and F. solani induced different patterns of metabolite production over 10 days of incubation and induced production of five de novo compounds not occurring in monocultures. These results emphasize that co-culture in time-frame analysis is an interesting method to unravel hidden metabolome in the investigation of fungal chemodiversity.
Identifiants
pubmed: 33289281
doi: 10.1002/cbdv.202000672
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000672Subventions
Organisme : Ministry of Research
Organisme : Technology and Higher Education of Republic Indonesia
Informations de copyright
© 2020 Wiley-VHCA AG, Zurich, Switzerland.
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