Analysis of differentially expressed genes in oral epithelial cells infected with Fusobacterium nucleatum for revealing genes associated with oral cancer.
Cell Line, Transformed
Cell Movement
/ genetics
Databases, Genetic
Epithelial Cells
/ metabolism
Fusobacterium nucleatum
/ physiology
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Gene Ontology
Gene Regulatory Networks
Genes, Neoplasm
Humans
Models, Biological
Mouth
/ pathology
Mouth Neoplasms
/ genetics
Protein Interaction Maps
/ genetics
RNA, Long Noncoding
/ genetics
RNA, Messenger
/ genetics
Reproducibility of Results
Fusobacterium nucleatum
TAGs
high-throughput sequencing
lncRNA
malignant transformation
Journal
Journal of cellular and molecular medicine
ISSN: 1582-4934
Titre abrégé: J Cell Mol Med
Pays: England
ID NLM: 101083777
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
07
06
2020
revised:
12
11
2020
accepted:
17
11
2020
pubmed:
9
12
2020
medline:
15
9
2021
entrez:
8
12
2020
Statut:
ppublish
Résumé
Accumulating evidence links Fusobacterium nucleatum with tumorigenesis. Our previous study demonstrated that F. nucleatum infection can induce epithelial-mesenchymal transition (EMT) in oral epithelial cells and elaborated a probable signal pathway involved in the induction of EMT. However, the comprehensive profiling and pathways of other candidate genes involved in F. nucleatum promoting malignant transformation remain largely elusive. Here, we analysed the transcriptome profile of HIOECs exposed to F. nucleatum infection. Totally, 3307 mRNAs (ǀLog2FCǀ >1.5) and 522 lncRNAs (ǀLog2FCǀ >1) were identified to be differentially expressed in F. nucleatum-infected HIOECs compared with non-infected HIOECs. GO and KEGG pathway analyses were performed to investigate the potential functions of the dysregulated genes. Tumour-associated genes were integrated, and top 10 hub genes (FYN, RAF1, ATM, FOS, CREB, NCOA3, VEGFA, JAK2, CREM and ATF3) were identified by protein-protein interaction (PPI) network, and Oncomine was used to validate hub genes' expression. LncRNA-hub genes co-expression network comprising 67 dysregulated lncRNAs were generated. Together, our study revealed the alteration of lncRNA and potential hub genes in oral epithelial cells in response to F. nucleatum infection, which may provide new insights into the shift of normal to malignant transformation initiated by oral bacterial infection.
Identifiants
pubmed: 33289330
doi: 10.1111/jcmm.16142
pmc: PMC7812288
doi:
Substances chimiques
RNA, Long Noncoding
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
892-904Subventions
Organisme : National Natural Science Foundation of China
ID : 81670997
Informations de copyright
© 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
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