Matrix stiffness and shear stresses modulate hepatocyte functions in a fibrotic liver sinusoidal model.
Albumins
/ metabolism
Animals
Cells, Cultured
Cytochrome P-450 Enzyme System
/ metabolism
Dimethylpolysiloxanes
/ chemistry
Extracellular Matrix
/ chemistry
Hepatocyte Nuclear Factor 4
/ metabolism
Hepatocytes
/ metabolism
Liver Cirrhosis
/ metabolism
Mice
Mice, Inbred C57BL
Microfluidics
/ instrumentation
Primary Cell Culture
/ methods
Stress, Mechanical
Tissue Scaffolds
/ chemistry
hepatocytes
liver fibrosis
matrix stiffness
shear flow
three-dimensional liver sinusoidal model
Journal
American journal of physiology. Gastrointestinal and liver physiology
ISSN: 1522-1547
Titre abrégé: Am J Physiol Gastrointest Liver Physiol
Pays: United States
ID NLM: 100901227
Informations de publication
Date de publication:
01 03 2021
01 03 2021
Historique:
pubmed:
10
12
2020
medline:
25
12
2021
entrez:
9
12
2020
Statut:
ppublish
Résumé
Extracellular matrix (ECM) rigidity has important effects on cell behaviors and increases sharply in liver fibrosis and cirrhosis. Hepatic blood flow is essential in maintaining hepatocytes' (HCs) functions. However, it is still unclear how matrix stiffness and shear stresses orchestrate HC phenotype in concert. A fibrotic three-dimensional (3-D) liver sinusoidal model is constructed using a porous membrane sandwiched between two polydimethylsiloxane (PDMS) layers with respective flow channels. The HCs are cultured in collagen gels of various stiffnesses in the lower channel, whereas the upper channel is pre-seeded with liver sinusoidal endothelial cells (LSECs) and accessible to shear flow. The results reveal that HCs cultured within stiffer matrices exhibit reduced albumin production and cytochrome
Identifiants
pubmed: 33296275
doi: 10.1152/ajpgi.00379.2019
pmc: PMC8609567
doi:
Substances chimiques
Albumins
0
Dimethylpolysiloxanes
0
HNF4A protein, human
0
Hepatocyte Nuclear Factor 4
0
baysilon
63148-62-9
Cytochrome P-450 Enzyme System
9035-51-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
G272-G282Références
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