CRISPR-Cas9-mediated 75.5-Mb inversion in maize.
Journal
Nature plants
ISSN: 2055-0278
Titre abrégé: Nat Plants
Pays: England
ID NLM: 101651677
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
24
07
2020
accepted:
04
11
2020
entrez:
10
12
2020
pubmed:
11
12
2020
medline:
4
5
2021
Statut:
ppublish
Résumé
CRISPR-Cas is a powerful double-strand-break technology with wide-ranging applications from gene discovery to commercial product development. Thus far, this tool has been almost exclusively used for gene knockouts and deletions, with a few examples of gene edits and targeted gene insertions. Here, we demonstrate the application of CRISPR-Cas9 technology to mediate targeted 75.5-Mb pericentric inversion in chromosome 2 in one of the elite maize inbred lines from Corteva Agriscience. This inversion unlocks a large chromosomal region containing substantial genetic variance for recombination, thus providing opportunities for the development of new maize varieties with improved phenotypes.
Identifiants
pubmed: 33299151
doi: 10.1038/s41477-020-00817-6
pii: 10.1038/s41477-020-00817-6
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1427-1431Références
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