Genomic characterisation of bovine papillomavirus types 1 and 2 identified in equine sarcoids in Japan.
bovine papillomavirus type 1
bovine papillomavirus type 2
equine sarcoid
horse
phylogenetic analysis
sequence
Journal
Equine veterinary journal
ISSN: 2042-3306
Titre abrégé: Equine Vet J
Pays: United States
ID NLM: 0173320
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
revised:
07
09
2020
received:
01
06
2020
accepted:
26
11
2020
pubmed:
11
12
2020
medline:
8
10
2021
entrez:
10
12
2020
Statut:
ppublish
Résumé
Bovine papillomavirus types 1 and 2 (BPV1/2) infection in horses has been associated with the development of equine sarcoids. Previous findings revealed the presence of sarcoid-associated BPV sequence variants that have been proposed as a key factor of cross-species infection in horses. To verify this hypothesis, sarcoid-associated BPV variants should be identified regardless of geographic location. Sequence analyses of BPV1/2 derived from both horses and cattle were conducted to clarify the sarcoid-associated sequence variants. The aim of this study was to clarify the correlation between BPV phylogeny and the geographic origin/host species. Cross-sectional study. Conventional PCR to detect BPV1/2 was performed with genomic DNA extracted from equine sarcoid (n = 10) and bovine papilloma (n = 10) samples collected in Japan. Direct sequencing results were compared between equine and bovine (equine/bovine)-derived BPV to identify sarcoid-associated variants of two early regions (E2, E5), one late region (L1) and the long control region (LCR). Phylogenetic and phylogeny-trait correlation were analysed using Bayesian Markov chain Monte Carlo (MCMC) method and Bayesian tip-association significance testing (BaTS). Seven BPV1 and three BPV2 were identified from equine sarcoids using PCR and direct sequencing. Sequence analysis of equine/bovine-derived samples showed no sarcoid-associated variants in four regions (E2, E5, L1 and LCR) of either BPV1 or BPV2. The phylogenetic tree of BPV1 E2, L1 and LCR tended to cluster within its geographic origins. BaTS analysis demonstrated that BPV1 sequence variability may be due to the geographic origin rather than host species difference. There was a limitation in sample numbers. This study supports the geographic-specific hypothesis of sequence variability, suggesting that BPV1 is shared between local equids and bovids. However, more extensively collected sequences worldwide and functional evaluations are needed to verify the geographic-specific sequence variability of BPV1/2 between equine- and bovine-derived sequence.
Sections du résumé
BACKGROUND
BACKGROUND
Bovine papillomavirus types 1 and 2 (BPV1/2) infection in horses has been associated with the development of equine sarcoids. Previous findings revealed the presence of sarcoid-associated BPV sequence variants that have been proposed as a key factor of cross-species infection in horses. To verify this hypothesis, sarcoid-associated BPV variants should be identified regardless of geographic location.
OBJECTIVES
OBJECTIVE
Sequence analyses of BPV1/2 derived from both horses and cattle were conducted to clarify the sarcoid-associated sequence variants. The aim of this study was to clarify the correlation between BPV phylogeny and the geographic origin/host species.
STUDY DESIGN
METHODS
Cross-sectional study.
METHODS
METHODS
Conventional PCR to detect BPV1/2 was performed with genomic DNA extracted from equine sarcoid (n = 10) and bovine papilloma (n = 10) samples collected in Japan. Direct sequencing results were compared between equine and bovine (equine/bovine)-derived BPV to identify sarcoid-associated variants of two early regions (E2, E5), one late region (L1) and the long control region (LCR). Phylogenetic and phylogeny-trait correlation were analysed using Bayesian Markov chain Monte Carlo (MCMC) method and Bayesian tip-association significance testing (BaTS).
RESULTS
RESULTS
Seven BPV1 and three BPV2 were identified from equine sarcoids using PCR and direct sequencing. Sequence analysis of equine/bovine-derived samples showed no sarcoid-associated variants in four regions (E2, E5, L1 and LCR) of either BPV1 or BPV2. The phylogenetic tree of BPV1 E2, L1 and LCR tended to cluster within its geographic origins. BaTS analysis demonstrated that BPV1 sequence variability may be due to the geographic origin rather than host species difference.
MAIN LIMITATIONS
CONCLUSIONS
There was a limitation in sample numbers.
CONCLUSIONS
CONCLUSIONS
This study supports the geographic-specific hypothesis of sequence variability, suggesting that BPV1 is shared between local equids and bovids. However, more extensively collected sequences worldwide and functional evaluations are needed to verify the geographic-specific sequence variability of BPV1/2 between equine- and bovine-derived sequence.
Substances chimiques
DNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1199-1209Subventions
Organisme : University of Tokyo
Informations de copyright
© 2020 EVJ Ltd.
Références
Rector A, Van Ranst M. Animal papillomaviruses. Virology. 2013;445:213-23.
Doorbar J, Quint W, Banks L, Bravo IG, Stoler M, Broker TR, et al. The biology and life-cycle of human papillomaviruses. Vaccine. 2012;30:55-70.
VanDoorslaer K, Chen Z, Bernard HU, Chan PKS, Desalle R, Dillner J, et al. ICTV virus taxonomy profile: papillomaviridae. J Gen Virol. 2018;99:989-90.
Maxie G. Jubb, Kennedy & Palmer’s Pathology of Domestic Animals, 6th edn. Philadelphia, USSA: Saunders Ltd.; 2015.
Ragland WL, Keown GH, Spencer GR. Equine sarcoid. Equine Vet J. 1996;2:302-4.
Knowles EJ, Tremaine WH, Pearson GR, Mair TS. A database survey of equine tumours in the United Kingdom. Equine Vet J. 2016;48:280-4.
Nasir L, Campo MS. Bovine papillomaviruses: their role in the aetiology of cutaneous tumours of bovids and equids. Vet Dermatol. 2008;19:243-54.
Nasir L, Brandt S. Papillomavirus associated diseases of the horse. Vet Microbiol. 2013;167:159-67.
Chambers G, Ellsmore VA, O’Brien PM, Reid SWJ, Love S, Campo MS, et al. Association of bovine papillomavirus with the equine sarcoid. J Gen Virol. 2003;84:1055-62.
Hartl B, Hainisch EK, Shafti-Keramat S, Kirnbauer R, Corteggio A, Borzacchiello G, et al. Inoculation of young horses with bovine papillomavirus type 1 virions leads to early infection of PBMCs prior to pseudo-sarcoid formation. J Gen Virol. 2011;92:2437-45.
Trewby H, Ayele G, Borzacchiello G, Brandt S, Campo MS, Fava CD, et al. Analysis of the long control region of bovine papillomavirus type 1 associated with sarcoids in equine hosts indicates multiple cross-species transmission events and phylogeographical structure. J Gen Virol. 2014;95:2748.
Nasir L, Gault E, Morgan IM, Chambers G, Ellsmore V, Campo MS. Identification and functional analysis of sequence variants in the long control region and the E2 open reading frame of bovine papillomavirus type 1 isolated from equine sarcoids. Virology. 2007;364:355-61.
Chambers G, Ellsmore VA, O’Brien PM, Reid SWJ, Love S, Campo MS, et al. Sequence variants of bovine papillomavirus E5 detected in equine sarcoids. Virus Res. 2003;96:141-5.
Savini F, Gallina L, Prosperi A, Battilani M, Bettini G, Scagliarini A. E5 nucleotide polymorphisms suggest quasispecies occurrence in BPV-1 sub-clinically infected horses. Res Vet Sci. 2015;102:80-2.
Koch C, Ramsauer AS, Drögemüller M, Ackermann M, Gerber V, Tobler K. Genomic comparison of bovine papillomavirus 1 isolates from bovine, equine and asinine lesional tissue samples. Virus Res. 2018;244:6-12.
Szczerba-Turek A, Siemionek J, Bancerz-Kisiel A, Raś A, Szweda W. Phylogenetic analysis of bovine papillomavirus E5 detected in equine sarcoids in Poland. Pol J Vet Sci. 2011;14:653-4.
Wilson AD, Armstrong ELR, Gofton RG, Mason J, Toit ND, Day MJ. Characterisation of early and late bovine papillomavirus protein expression in equine sarcoids. Vet Microbiol. 2013;162:369-80.
Yamashita-Kawanishi N, Tsuzuki M, Wei Z, Kok MK, Ishiyama D, Chambers JK, et al. Identification of bovine papillomavirus type 1 and 2 from bovine anogenital fibropapillomas. J Vet Med Sci. 2019;81:1000-5.
Arreaza G, Qiu P, Pang L, Albright A, Hong L, Marton M, et al. Pre-analytical considerations for successful next-generation sequencing (NGS): challenges and opportunities for formalin-fixed and paraffin-embedded tumor tissue (FFPE) samples. Int J Mol Sci. 2016;17:1579.
Kawauchi K, Takahashi C, Ishihara R, Hatama S. Development of a novel PCR-RFLP assay for improved detection and typing of bovine papillomaviruses. J Virol Methods. 2015;218:23-6.
Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33:1870-4.
Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 2018;35:1547-9.
Godi A, Bissett SL, Masloh S, Fleury M, Li S, Zhao Q, et al. Impact of naturally occurring variation in the human papillomavirus 52 capsid proteins on recognition by type-specific neutralising antibodies. J Gen Virol. 2019;100:237-45.
Shimakura H, Dong J, Zhu W, Chambers JK, Uchida K, Kiriki K, et al. Full genome analysis of bovine papillomavirus type 1 derived from a calf with severe cutaneous multiple papillomatosis. J Vet Med Sci. 2018;11:1691-5.
Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994;22:4673-80.
Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, et al. Mrbayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space. Syst Biol. 2012;61:539-42.
Rector A, Lemey P, Tachezy R, Mostmans S, Ghim S-J, Doorslaer KV, et al. Ancient papillomavirus-host co-speciation in Felidae. Genome Biol. 2007;8(4):1-12.
Parker J, Rambaut A, Pybus OG. Correlating viral phenotypes with phylogeny: accounting for phylogenetic uncertainty. Infect Genet Evol. 2008;8:239-46.
Sievers F, Higgins DG. Clustal Omega for making accurate alignments of many protein sequences. Protein Sci. 2018;27:135-45.
Mohammed HO, Rebhun WC, Antczak DF. Factors associated with the risk of developing sarcoid tumours in horses. Equine Vet J. 1992;24:165-8.
Petti L, DiMaio D. Specific interaction between the bovine papillomavirus E5 transforming protein and the beta receptor for platelet-derived growth factor in stably transformed and acutely transfected cells. J Virol. 1994;68:3582-92.