Morphine-mediated release of miR-138 in astrocyte-derived extracellular vesicles promotes microglial activation.
drug abuse
extracellular vesicles
miRNA
microglia
neuroinflammation
opioids
Journal
Journal of extracellular vesicles
ISSN: 2001-3078
Titre abrégé: J Extracell Vesicles
Pays: United States
ID NLM: 101610479
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
07
05
2020
revised:
20
09
2020
accepted:
15
10
2020
entrez:
11
12
2020
pubmed:
12
12
2020
medline:
12
12
2020
Statut:
ppublish
Résumé
Opioids, such as morphine, are the mainstay for the management of postsurgical pain. Over the last decade there has been a dramatic increase in deaths related to opioid overdose. While opioid abuse has been shown to result in increased neuroinflammation, mechanism(s) underlying this process, remain less understood. In recent years, microRNAs have emerged as key mediators of gene expression regulating both paracrine signaling and cellular crosstalk. MiRNAs constitute the extracellular vesicle (EV) cargo and can shuttle from the donor to the recipient cells. Exposure of human primary astrocytes to morphine resulted in induction and release of miR-138 in the EVs isolated from conditioned media of cultured astrocytes. Released EVs were, in turn, taken up by the microglia, leading to activation of these latter cells. Interestingly, activation of microglia involved binding of the GUUGUGU motif of miR138 to the endosomal toll like receptor (TLR)7, leading, in turn, to cellular activation. These findings were further corroborated in vivo in wildtype mice wherein morphine administration resulted in increased microglial activation in the thalamus. In TLR7
Identifiants
pubmed: 33304479
doi: 10.1002/jev2.12027
pii: JEV212027
pmc: PMC7710131
doi:
Substances chimiques
MIRN138 microRNA, mouse
0
Membrane Glycoproteins
0
MicroRNAs
0
Tlr7 protein, mouse
0
Toll-Like Receptor 7
0
Morphine
76I7G6D29C
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
e12027Subventions
Organisme : NIMH NIH HHS
ID : P30 MH062261
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA040397
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA041751
Pays : United States
Informations de copyright
© 2020 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.
Déclaration de conflit d'intérêts
The authors declare no competing financial interests in relation to the work described.
Références
Front Immunol. 2020 Mar 10;11:406
pubmed: 32210973
Nat Commun. 2019 Dec 11;10(1):5656
pubmed: 31827089
J Neurosci. 1982 Aug;2(8):1129-49
pubmed: 6286904
FEBS Open Bio. 2016 Jun 14;6(8):835-46
pubmed: 27516962
Clin Chim Acta. 2019 Oct;497:27-34
pubmed: 31301281
Neurobiol Aging. 2019 Feb;74:15-20
pubmed: 30396120
Int J Mol Sci. 2019 Jan 20;20(2):
pubmed: 30669512
J Neuroinflammation. 2014 Apr 03;11:68
pubmed: 24694258
Neuroscience. 2019 May 1;405:148-157
pubmed: 29660443
Brain Behav Immun. 2009 Feb;23(2):240-50
pubmed: 18938237
J Neuroinflammation. 2019 Jul 4;16(1):136
pubmed: 31272469
AIDS Res Hum Retroviruses. 2012 Oct;28(10):1313-28
pubmed: 22280204
J Neuroinflammation. 2016 Feb 09;13:33
pubmed: 26860188
Epigenetics. 2016 Nov;11(11):819-830
pubmed: 27786595
J Extracell Vesicles. 2019 Apr 14;8(1):1599680
pubmed: 31044053
J Neuroimmune Pharmacol. 2018 Sep;13(3):330-344
pubmed: 29497921
Acta Neuropathol. 2018 Jul;136(1):41-56
pubmed: 29934873
Sci Rep. 2018 Feb 26;8(1):3596
pubmed: 29483538
J Vis Exp. 2018 Jun 5;(136):
pubmed: 29939190
J Neuroimmune Pharmacol. 2017 Sep;12(3):420-432
pubmed: 28236278
Prog Neurobiol. 2019 Dec;183:101694
pubmed: 31542363
Mol Neurobiol. 2018 Apr;55(4):3196-3210
pubmed: 28478506
Acta Neuropathol. 2017 Feb;133(2):303-319
pubmed: 27888296
J Neurosci. 2014 Sep 17;34(38):12850-64
pubmed: 25232120
Am J Cardiol. 1966 Dec;18(6):876-83
pubmed: 5923998
Cells. 2020 Jul 04;9(7):
pubmed: 32635578
Nat Immunol. 2001 Aug;2(8):675-80
pubmed: 11477402
Theranostics. 2018 Apr 9;8(10):2709-2721
pubmed: 29774070
Science. 2004 Mar 5;303(5663):1526-9
pubmed: 14976262
Front Cell Neurosci. 2019 Feb 14;13:51
pubmed: 30837842
Clin J Pain. 2018 Sep;34(9):878-884
pubmed: 29505419
CNS Neurol Disord Drug Targets. 2014;13(9):1542-58
pubmed: 25106635
Cancer Lett. 2020 Jan 28;469:186-194
pubmed: 31669517
BMC Genomics. 2018 Feb 14;19(1):146
pubmed: 29444662
J Virol. 2015 Jan 15;89(2):1024-35
pubmed: 25355898
Immunity. 2016 Oct 18;45(4):737-748
pubmed: 27742543
Mol Aspects Med. 2018 Apr;60:115-122
pubmed: 29122679
Nat Commun. 2019 Sep 12;10(1):4136
pubmed: 31515491
Cell. 2016 Mar 10;164(6):1226-1232
pubmed: 26967288
Sci Rep. 2020 Jan 21;10(1):828
pubmed: 31964978
Exp Neurol. 1984 Jan;83(1):118-33
pubmed: 6537810
Cancer Med. 2019 Jun;8(6):3142-3151
pubmed: 31063269
J Anim Sci. 1990 Jun;68(6):1505-12
pubmed: 2384351
J Neuroimmunol. 2017 May 15;306:1-10
pubmed: 28385180
Neuroscience. 2001;102(3):555-63
pubmed: 11226693
Front Cell Neurosci. 2018 Oct 16;12:306
pubmed: 30459555
Front Cell Neurosci. 2017 Aug 08;11:235
pubmed: 28848398
Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):E2110-6
pubmed: 22753494
Mol Ther Nucleic Acids. 2018 Dec 7;13:450-463
pubmed: 30388619
Psychopharmacology (Berl). 2014 Jul;231(13):2623-32
pubmed: 24448901
J Cell Biol. 2016 Oct 24;215(2):245-258
pubmed: 27810915
Ann Transl Med. 2015 Jun;3(10):136
pubmed: 26207229
Nano Lett. 2018 Jul 11;18(7):4226-4232
pubmed: 29888919
Cell Death Dis. 2018 Mar 5;9(3):363
pubmed: 29507357
Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10230-4
pubmed: 8234282
Neuropsychopharmacology. 2018 Dec;43(13):2606-2614
pubmed: 30258112
Anesth Analg. 2016 Dec;123(6):1429-1436
pubmed: 27167686
Cell. 2003 Jun 13;113(6):673-6
pubmed: 12809598
PLoS Pathog. 2015 Jul 08;11(7):e1005032
pubmed: 26154133
Proc Natl Acad Sci U S A. 2019 Jul 2;116(27):13523-13532
pubmed: 31209039
Front Cell Neurosci. 2019 Oct 25;13:472
pubmed: 31708746
J Cell Mol Med. 2017 May;21(5):986-992
pubmed: 28224705
J Neurosci. 2012 Jul 18;32(29):9917-30
pubmed: 22815507
Immunology. 2020 Jul;160(3):269-279
pubmed: 32053234
Cell. 2009 Jan 23;136(2):215-33
pubmed: 19167326
J Neuroimmune Pharmacol. 2020 Sep;15(3):538-553
pubmed: 31401755
Pediatr Res. 2003 Aug;54(2):282-8
pubmed: 12736382
Nat Rev Genet. 2008 Feb;9(2):102-14
pubmed: 18197166
Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7242-6
pubmed: 7638174
Curr HIV Res. 2012 Jul;10(5):469-77
pubmed: 22591371
J Extracell Vesicles. 2020 Oct;10(1):e12027
pubmed: 33304479
Int J Cancer. 2016 Oct 1;139(7):1443-8
pubmed: 27170104
Front Pharmacol. 2019 Dec 02;10:1452
pubmed: 31849688
Pain Rep. 2018 Feb 23;3(2):e640
pubmed: 29756086
Mol Cancer. 2018 Oct 11;17(1):147
pubmed: 30309355
FEMS Immunol Med Microbiol. 2012 Mar;64(2):228-36
pubmed: 22066570
Neurobiol Dis. 2016 Aug;92(Pt B):124-36
pubmed: 26845176
Exp Ther Med. 2019 Oct;18(4):2540-2546
pubmed: 31572505
AIDS Res Treat. 2012;2012:953678
pubmed: 22666564
Neurotoxicology. 2020 Mar;77:181-192
pubmed: 32014511
PLoS One. 2008;3(12):e4093
pubmed: 19116667
Int J Mol Sci. 2020 Mar 27;21(7):
pubmed: 32230793
Drugs Context. 2018 Apr 11;7:212524
pubmed: 29675049
Int J Oncol. 2019 Feb;54(2):713-721
pubmed: 30483768
Acta Neuropathol. 2014 Nov;128(5):639-650
pubmed: 24997849
Nat Neurosci. 2012 Jun;15(6):827-35
pubmed: 22610069
Cell. 2004 Jan 23;116(2):281-97
pubmed: 14744438
Front Behav Neurosci. 2014 Apr 03;8:117
pubmed: 24765071
Sci Rep. 2015 May 21;5:10501
pubmed: 25994622
J Biol Chem. 2016 Jan 15;291(3):1251-66
pubmed: 26553869
Arch Intern Med. 2012 Mar 12;172(5):425-30
pubmed: 22412106
ACS Nano. 2018 Apr 24;12(4):3311-3320
pubmed: 29570265
Cell Death Dis. 2012 Aug 30;3:e381
pubmed: 22932723