Bovine pyruvate carboxylase gene proximal promoter activity is regulated by saturated and unsaturated fatty acids in Madin-Darby bovine kidney cells.
bovine
fatty acid
pyruvate carboxylase proximal promoter
Journal
Journal of dairy science
ISSN: 1525-3198
Titre abrégé: J Dairy Sci
Pays: United States
ID NLM: 2985126R
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
28
04
2020
accepted:
17
09
2020
pubmed:
15
12
2020
medline:
16
3
2021
entrez:
14
12
2020
Statut:
ppublish
Résumé
An increase in bovine pyruvate carboxylase (PC; EC 6.4.1.1) at calving and during feed restriction corresponds with increased circulating nonesterified fatty acids as a consequence of negative energy balance. Regulation of PC mRNA and effect of specific combinations of saturated and unsaturated fatty acid profiles has yet to be explored. Our objective was to determine the effects of chain length, degree of saturation, and copresence of saturated and unsaturated fatty acids on activity of bovine PC promoter 1 (PCP1). For these experiments, Madin-Darby bovine kidney cells were transfected with a full-length bovine PCP1 construct from -1002 to +3 bp relative to the bovine PC gene transcription start site (bovine PCP1
Identifiants
pubmed: 33309342
pii: S0022-0302(20)31014-6
doi: 10.3168/jds.2020-18803
pii:
doi:
Substances chimiques
Fatty Acids
0
Fatty Acids, Unsaturated
0
PPAR alpha
0
Pyruvate Carboxylase
EC 6.4.1.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2308-2317Informations de copyright
Copyright © 2021 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.