Tracking and interpreting long-range chromatin interactions with super-resolution live-cell imaging.
3D genome
CTCF
Chromatin looping
Cohesin
Dynamics
Enhancers
Gene regulation
Microscopy
SRLCI
Super-resolution live-cell imaging
Journal
Current opinion in cell biology
ISSN: 1879-0410
Titre abrégé: Curr Opin Cell Biol
Pays: England
ID NLM: 8913428
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
01
10
2020
revised:
03
11
2020
accepted:
06
11
2020
pubmed:
15
12
2020
medline:
26
10
2021
entrez:
14
12
2020
Statut:
ppublish
Résumé
Mammalian genomes are organized and regulated through long-range chromatin interactions. Structural loops formed by CCCTC-binding factor (CTCF) and cohesin fold the genome into domains, while enhancers interact with promoters across vast genomic distances to regulate gene expression. Although genomics and fixed-cell imaging approaches help illuminate many aspects of chromatin interactions, temporal information is usually lost. Here, we discuss how 3D super-resolution live-cell imaging (SRLCI) can resolve open questions on the dynamic formation and dissolution of chromatin interactions. We discuss SRLCI experimental design, implementation strategies, and data interpretation and highlight associated pitfalls. We conclude that, while technically demanding, SRLCI approaches will likely emerge as a critical tool to dynamically probe 3D genome structure and function and to study enhancer-promoter interactions and chromatin looping.
Identifiants
pubmed: 33310227
pii: S0955-0674(20)30152-6
doi: 10.1016/j.ceb.2020.11.002
pmc: PMC8364313
mid: NIHMS1732157
pii:
doi:
Substances chimiques
CCCTC-Binding Factor
0
Cell Cycle Proteins
0
Chromatin
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
18-26Subventions
Organisme : NIGMS NIH HHS
ID : DP2 GM140938
Pays : United States
Organisme : NIGMS NIH HHS
ID : R00 GM130896
Pays : United States
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest statement Nothing declared.
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