Hairpin-bisulfite sequencing of cells exposed to decitabine documents the process of DNA demethylation.
Demethylation
decitabine
dnmt1 inhibition
Journal
Epigenetics
ISSN: 1559-2308
Titre abrégé: Epigenetics
Pays: United States
ID NLM: 101265293
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
pubmed:
15
12
2020
medline:
13
1
2022
entrez:
14
12
2020
Statut:
ppublish
Résumé
Although the mechanism of DNA demethylating drugs has been understood for many years, the direct effect of these drugs on methylation of the complementary strands of DNA has not been formally demonstrated. By using hairpin-bisulphite sequencing, we describe the kinetics and pattern of DNA methylation following treatment of cells by the DNA methyltransferase 1 (DNMT1) inhibitor, decitabine. As expected, we demonstrate complete loss of methylation on the daughter strand following S-phase in selected densely methylated genes in synchronized Jurkat cells. Thereafter, cells showed a heterogeneous pattern of methylation reflecting replication of the unmethylated strand and restoration of methylation.
Identifiants
pubmed: 33315501
doi: 10.1080/15592294.2020.1861169
pmc: PMC8813075
doi:
Substances chimiques
Sulfites
0
Decitabine
776B62CQ27
DNA (Cytosine-5-)-Methyltransferase 1
EC 2.1.1.37
DNA (Cytosine-5-)-Methyltransferases
EC 2.1.1.37
Azacitidine
M801H13NRU
hydrogen sulfite
OJ9787WBLU
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1251-1259Références
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