SNAP23 deficiency causes severe brain dysplasia through the loss of radial glial cell polarity.
Animals
Apoptosis
Brain
/ pathology
COS Cells
Cadherins
/ metabolism
Cell Differentiation
Cell Membrane
/ metabolism
Cell Movement
Cell Nucleus
/ metabolism
Cell Polarity
Cells, Cultured
Chlorocebus aethiops
Down-Regulation
Gait
Mice, Knockout
Neurogenesis
Neuroglia
/ metabolism
Neurons
/ pathology
Qb-SNARE Proteins
/ deficiency
Qc-SNARE Proteins
/ deficiency
R-SNARE Proteins
Receptors, Notch
/ metabolism
Signal Transduction
Syntaxin 1
/ metabolism
Transport Vesicles
/ metabolism
beta Catenin
/ metabolism
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
04 01 2021
04 01 2021
Historique:
received:
13
10
2019
revised:
23
08
2020
accepted:
23
10
2020
entrez:
17
12
2020
pubmed:
18
12
2020
medline:
19
5
2021
Statut:
ppublish
Résumé
In the developing brain, the polarity of neural progenitor cells, termed radial glial cells (RGCs), is important for neurogenesis. Intercellular adhesions, termed apical junctional complexes (AJCs), at the apical surface between RGCs are necessary for cell polarization. However, the mechanism by which AJCs are established remains unclear. Here, we show that a SNARE complex composed of SNAP23, VAMP8, and Syntaxin1B has crucial roles in AJC formation and RGC polarization. Central nervous system (CNS)-specific ablation of SNAP23 (NcKO) results in mice with severe hypoplasia of the neocortex and no hippocampus or cerebellum. In the developing NcKO brain, RGCs lose their polarity following the disruption of AJCs and exhibit reduced proliferation, increased differentiation, and increased apoptosis. SNAP23 and its partner SNAREs, VAMP8 and Syntaxin1B, are important for the localization of an AJC protein, N-cadherin, to the apical plasma membrane of RGCs. Altogether, SNARE-mediated localization of N-cadherin is essential for AJC formation and RGC polarization during brain development.
Identifiants
pubmed: 33332551
pii: 211605
doi: 10.1083/jcb.201910080
pmc: PMC7754684
pii:
doi:
Substances chimiques
Cadherins
0
Qb-SNARE Proteins
0
Qc-SNARE Proteins
0
R-SNARE Proteins
0
Receptors, Notch
0
Snap23 protein, mouse
0
Syntaxin 1
0
Vamp8 protein, mouse
0
beta Catenin
0
syntaxin 1B, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 Kunii et al.
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