A single cell atlas of the human liver tumor microenvironment.
human cell atlas
liver cancer
single cell RNAseq
spatial transcriptomics
tumor-stroma interactions
Journal
Molecular systems biology
ISSN: 1744-4292
Titre abrégé: Mol Syst Biol
Pays: England
ID NLM: 101235389
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
01
05
2020
revised:
11
11
2020
accepted:
18
11
2020
entrez:
17
12
2020
pubmed:
18
12
2020
medline:
27
8
2021
Statut:
ppublish
Résumé
Malignant cell growth is fueled by interactions between tumor cells and the stromal cells composing the tumor microenvironment. The human liver is a major site of tumors and metastases, but molecular identities and intercellular interactions of different cell types have not been resolved in these pathologies. Here, we apply single cell RNA-sequencing and spatial analysis of malignant and adjacent non-malignant liver tissues from five patients with cholangiocarcinoma or liver metastases. We find that stromal cells exhibit recurring, patient-independent expression programs, and reconstruct a ligand-receptor map that highlights recurring tumor-stroma interactions. By combining transcriptomics of laser-capture microdissected regions, we reconstruct a zonation atlas of hepatocytes in the non-malignant sites and characterize the spatial distribution of each cell type across the tumor microenvironment. Our analysis provides a resource for understanding human liver malignancies and may expose potential points of interventions.
Identifiants
pubmed: 33332768
doi: 10.15252/msb.20209682
pmc: PMC7746227
doi:
Banques de données
GEO
['GSE146409']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e9682Subventions
Organisme : Howard Hughes Medical Institute
Pays : United States
Informations de copyright
© 2020 The Authors. Published under the terms of the CC BY 4.0 license.
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