The Association of Scoliosis and NSD1 Gene Deletion in Sotos Syndrome Patients.


Journal

Spine
ISSN: 1528-1159
Titre abrégé: Spine (Phila Pa 1976)
Pays: United States
ID NLM: 7610646

Informations de publication

Date de publication:
01 Jul 2021
Historique:
pubmed: 18 12 2020
medline: 10 7 2021
entrez: 17 12 2020
Statut: ppublish

Résumé

A retrospective comparative study. The aim of this study was to examine the NSD1 abnormalities in patients diagnosed with Sotos syndrome and its correlation with the presence, severity, and progression of associated scoliosis. Scoliosis has been reported in approximately 30% of patients diagnosed with Sotos syndrome, a genetic disorder characterized by a distinctive facial appearance, learning disability, and overgrowth. Sotos syndrome is mainly attributed to NSD1 haploinsufficiency, but with ethnical differences in genetic profile: NSD1 microdeletions are frequently identified in Japanese Sotos patients whereas intragenic mutations are more frequently found in non-Japanese patients. Although possible genotype-phenotype correlations have been proposed, the genotype of Sotos syndrome patients suffering from scoliosis has not been examined. The medical records and spinal radiographs of 63 consecutive Sotos syndrome patients at a single center were reviewed. Fluorescent in situ hybridization or microarray comparative genomic hybridization and DNA sequencing or multiplex ligation-dependent probe amplification were performed to detect 5q35 microdeletion involving the NSD1 gene and intragenic mutations of the NSD1 gene, respectively. The phenotypes of all cases and radiological assessments for the presence and progression of scoliosis were studied. NSD1 abnormalities were identified in 55 patients (87%): microdeletion in 34 patients (54%) and intragenic mutation in 22 patients (33%). Scoliosis was observed in 26 patients (41%), with a significantly higher ratio of microdeletions than mutations. The 10 patients with progressive scoliosis all had NSD1 microdeletions. Scoliosis was a common phenotypical trait in children with Sotos syndrome and its presence as well as progression were higher in cases with NSD1 microdeletions. Although all Sotos syndrome patients should be monitored for scoliosis, clinicians should be made aware that patients with NSD1 microdeletions have a higher probability of scoliosis development and progression that may require early intervention.Level of Evidence: 3.

Sections du résumé

STUDY DESIGN METHODS
A retrospective comparative study.
OBJECTIVE OBJECTIVE
The aim of this study was to examine the NSD1 abnormalities in patients diagnosed with Sotos syndrome and its correlation with the presence, severity, and progression of associated scoliosis.
SUMMARY OF BACKGROUND DATA BACKGROUND
Scoliosis has been reported in approximately 30% of patients diagnosed with Sotos syndrome, a genetic disorder characterized by a distinctive facial appearance, learning disability, and overgrowth. Sotos syndrome is mainly attributed to NSD1 haploinsufficiency, but with ethnical differences in genetic profile: NSD1 microdeletions are frequently identified in Japanese Sotos patients whereas intragenic mutations are more frequently found in non-Japanese patients. Although possible genotype-phenotype correlations have been proposed, the genotype of Sotos syndrome patients suffering from scoliosis has not been examined.
METHODS METHODS
The medical records and spinal radiographs of 63 consecutive Sotos syndrome patients at a single center were reviewed. Fluorescent in situ hybridization or microarray comparative genomic hybridization and DNA sequencing or multiplex ligation-dependent probe amplification were performed to detect 5q35 microdeletion involving the NSD1 gene and intragenic mutations of the NSD1 gene, respectively. The phenotypes of all cases and radiological assessments for the presence and progression of scoliosis were studied.
RESULTS RESULTS
NSD1 abnormalities were identified in 55 patients (87%): microdeletion in 34 patients (54%) and intragenic mutation in 22 patients (33%). Scoliosis was observed in 26 patients (41%), with a significantly higher ratio of microdeletions than mutations. The 10 patients with progressive scoliosis all had NSD1 microdeletions.
CONCLUSION CONCLUSIONS
Scoliosis was a common phenotypical trait in children with Sotos syndrome and its presence as well as progression were higher in cases with NSD1 microdeletions. Although all Sotos syndrome patients should be monitored for scoliosis, clinicians should be made aware that patients with NSD1 microdeletions have a higher probability of scoliosis development and progression that may require early intervention.Level of Evidence: 3.

Identifiants

pubmed: 33332788
doi: 10.1097/BRS.0000000000003879
pii: 00007632-202107010-00007
doi:

Substances chimiques

Histone-Lysine N-Methyltransferase EC 2.1.1.43
NSD1 protein, human EC 2.1.1.43

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

E726-E733

Informations de copyright

Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.

Références

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Tatton-Brown K, Douglas J, Coleman K, et al. Multiple mechanisms are implicated in the generation of 5q35 microdeletions in Sotos syndrome. J Med Genet 2005; 42:307–313.
Visser R, Hasegawa T, Niikawa N, et al. Analysis of the NSD1 promoter region in patients with a Sotos syndrome phenotype. J Hum Genet 2006; 51:15–20.
Douglas J, Hanks S, Temple IK, et al. NSD1 mutations are the major cause of Sotos syndrome and occur in some cases of Weaver syndrome but are rare in other overgrowth phenotypes. Am J Hum Genet 2003; 72:132–143.
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Auteurs

Masafumi Machida (M)

Department of Orthopedic Surgery, Saitama Children Medical Center, Saitama City, Saitama, Japan.

Hiroyuki Katoh (H)

Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, Isehara City, Kanagawa, Japan.

Masayoshi Machida (M)

Department of Orthopaedic Surgery, The Hospital for Sick Children, Toronto, Ontario, Canada.

Atsushi Miyake (A)

Department of Orthopaedic Surgery, National Defense Medical College, Tokorozawa City, Saitama, Japan.

Katsuaki Taira (K)

Department of Orthopedic Surgery, Saitama Children Medical Center, Saitama City, Saitama, Japan.

Hirofumi Ohashi (H)

Division of Medical Genetics, Saitama Children Medical Center, Saitama City, Saitama, Japan.

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Classifications MeSH