A Binary Cre Transgenic Approach Dissects Microglia and CNS Border-Associated Macrophages.
Lyve1 BAM
RiboTag
binary transgenic
brain macrophages
intersectional genetics
meninges
microglia
perivascular macrophages
pia mater
split cre
Journal
Immunity
ISSN: 1097-4180
Titre abrégé: Immunity
Pays: United States
ID NLM: 9432918
Informations de publication
Date de publication:
12 01 2021
12 01 2021
Historique:
received:
10
06
2020
revised:
09
08
2020
accepted:
16
11
2020
pubmed:
18
12
2020
medline:
10
9
2021
entrez:
17
12
2020
Statut:
ppublish
Résumé
The developmental and molecular heterogeneity of tissue macrophages is unravelling, as are their diverse contributions to physiology and pathophysiology. Moreover, also given tissues harbor macrophages in discrete anatomic locations. Functional contributions of specific cell populations can in mice be dissected using Cre recombinase-mediated mutagenesis. However, single promoter-based Cre models show limited specificity for cell types. Focusing on macrophages in the brain, we establish here a binary transgenic system involving complementation-competent NCre and CCre fragments whose expression is driven by distinct promoters: Sall1
Identifiants
pubmed: 33333014
pii: S1074-7613(20)30494-5
doi: 10.1016/j.immuni.2020.11.007
pii:
doi:
Substances chimiques
Cre recombinase
EC 2.7.7.-
Integrases
EC 2.7.7.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
176-190.e7Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare that they have no competing interests.