Effects mediated by the α7 nicotinic acetylcholine receptor on cell proliferation and migration in rat adipose-derived stem cells.
Journal
European journal of histochemistry : EJH
ISSN: 2038-8306
Titre abrégé: Eur J Histochem
Pays: Italy
ID NLM: 9207930
Informations de publication
Date de publication:
30 Oct 2020
30 Oct 2020
Historique:
received:
13
07
2020
accepted:
25
09
2020
entrez:
18
12
2020
pubmed:
19
12
2020
medline:
10
7
2021
Statut:
epublish
Résumé
Adipose-derived stem cells (ASCs) are an attractive source for regenerative medicine as they can be easily isolated, rapidly expandable in culture and show excellent in vitro differentiation potential. Acetylcholine (ACh), one of the main neurotransmitters in central and peripheral nervous systems, plays key roles in the control of several physiological processes also in non-neural tissues. As demonstrated in our previous studies, ACh can contribute to the rat ASCs physiology, negatively modulating ASCs proliferation and migration via M2 muscarinic receptor (mAChR) activation. In the present work we show that rat ASCs also express α7 nicotinic receptors (nAChRs). In particular, we have investigated the effects mediated by the selective activation of α7 nAChRs, which causes a reduction of ASC proliferation without affecting cell survival and morphology, and significantly promotes cell migration via upregulation of the CXCR4 expression. Interestingly, the activation of the α7 nAChR also upregulates the expression of M2 mAChR protein, indicating a cooperation between muscarinic and nicotinic receptors in the inhibition of ASC proliferation.
Identifiants
pubmed: 33334089
doi: 10.4081/ejh.2020.3159
pmc: PMC7643034
doi:
Substances chimiques
Bungarotoxins
0
Fumarates
0
ICH3 alpha7 nicotinic acetylcholine receptor agonist
0
Spiro Compounds
0
alpha7 Nicotinic Acetylcholine Receptor
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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