The p53-induced RNA-binding protein ZMAT3 is a splicing regulator that inhibits the splicing of oncogenic CD44 variants in colorectal carcinoma.
Alternative Splicing
/ genetics
Carcinogenesis
/ genetics
Colorectal Neoplasms
/ genetics
Gene Knockdown Techniques
Gene Silencing
HCT116 Cells
HEK293 Cells
Humans
Hyaluronan Receptors
/ genetics
Protein Binding
/ genetics
RNA Precursors
/ metabolism
RNA Splicing
/ genetics
RNA-Binding Proteins
/ genetics
Tumor Suppressor Protein p53
/ metabolism
CD44
PAR-CLIP
RBP
RNA-binding protein
ZMAT3
colorectal carcinoma
p53
splicing
Journal
Genes & development
ISSN: 1549-5477
Titre abrégé: Genes Dev
Pays: United States
ID NLM: 8711660
Informations de publication
Date de publication:
01 01 2021
01 01 2021
Historique:
received:
15
07
2020
accepted:
26
10
2020
pubmed:
19
12
2020
medline:
5
8
2021
entrez:
18
12
2020
Statut:
ppublish
Résumé
p53 is an intensely studied tumor-suppressive transcription factor. Recent studies suggest that the RNA-binding protein (RBP) ZMAT3 is important in mediating the tumor-suppressive effects of p53. Here, we globally identify ZMAT3-regulated RNAs and their binding sites at nucleotide resolution in intact colorectal cancer (CRC) cells. ZMAT3 binds to thousands of mRNA precursors, mainly at intronic uridine-rich sequences and affects their splicing. The strongest alternatively spliced ZMAT3 target was
Identifiants
pubmed: 33334821
pii: gad.342634.120
doi: 10.1101/gad.342634.120
pmc: PMC7778265
doi:
Substances chimiques
CD44 protein, human
0
Hyaluronan Receptors
0
RNA Precursors
0
RNA-Binding Proteins
0
Tumor Suppressor Protein p53
0
ZMAT3 protein, human
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
102-116Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM132458
Pays : United States
Organisme : NIA NIH HHS
ID : R21 AG065748
Pays : United States
Informations de copyright
© 2021 Muys et al.; Published by Cold Spring Harbor Laboratory Press.
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