The immunophilin Zonda controls regulated exocytosis in endocrine and exocrine tissues.
Drosophila
Exocytosis
Immunophilin
RalA
Salivary gland
Secretory granule
Zonda
Journal
Traffic (Copenhagen, Denmark)
ISSN: 1600-0854
Titre abrégé: Traffic
Pays: England
ID NLM: 100939340
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
28
06
2020
revised:
15
12
2020
accepted:
15
12
2020
pubmed:
19
12
2020
medline:
11
5
2021
entrez:
18
12
2020
Statut:
ppublish
Résumé
Exocytosis is a fundamental process in physiology, that ensures communication between cells, organs and even organisms. Hormones, neuropeptides and antibodies, among other cargoes are packed in exocytic vesicles that need to reach and fuse with the plasma membrane to release their content to the extracellular milieu. Hundreds of proteins participate in this process and several others in its regulation. We report here a novel component of the exocytic machinery, the Drosophila transmembrane immunophilin Zonda (Zda), previously found to participate in autophagy. Zda is highly expressed in secretory tissues, and regulates exocytosis in at least three of them: the ring gland, insulin-producing cells and the salivary gland. Using the salivary gland as a model system, we found that Zda is required at final steps of the exocytic process for fusion of secretory granules to the plasma membrane. In a genetic screen we identified the small GTPase RalA as a crucial regulator of secretory granule exocytosis that is required, similarly to Zda, for fusion between the secretory granule and the plasma membrane.
Substances chimiques
Immunophilins
EC 5.2.1.8
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
111-122Subventions
Organisme : Agencia Nacional de Promoción Científica y Tecnológica
ID : PICT 2011-0090
Organisme : Agencia Nacional de Promoción Científica y Tecnológica
ID : PICT 2011-2556
Organisme : Agencia Nacional de Promoción Científica y Tecnológica
ID : PICT 2012-0214
Organisme : Agencia Nacional de Promoción Científica y Tecnológica
ID : PICT 2012-2376
Organisme : Agencia Nacional de Promoción Científica y Tecnológica
ID : PICT 2015-0649
Organisme : Agencia Nacional de Promoción Científica y Tecnológica
ID : PICT 2017-1356
Organisme : Agencia Nacional de Promoción Científica y Tecnológica
ID : PICT 2018-1501
Informations de copyright
© 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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