Measuring acetyl-CoA and acetylated histone turnover in vivo: Effect of a high fat diet.


Journal

Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535

Informations de publication

Date de publication:
15 02 2021
Historique:
received: 10 08 2020
revised: 08 12 2020
accepted: 13 12 2020
pubmed: 20 12 2020
medline: 23 6 2021
entrez: 19 12 2020
Statut: ppublish

Résumé

Cellular availability of acetyl-CoA, a central intermediate of metabolism, regulates histone acetylation. The impact of a high-fat diet (HFD) on the turnover rates of acetyl-CoA and acetylated histones is unknown. We developed a method for simultaneous measurement of acetyl-CoA and acetylated histones kinetics using a single

Identifiants

pubmed: 33340539
pii: S0003-2697(20)30599-6
doi: 10.1016/j.ab.2020.114067
pii:
doi:

Substances chimiques

Histones 0
Acetyl Coenzyme A 72-89-9
Deuterium Oxide J65BV539M3
Lysine K3Z4F929H6

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

114067

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM112044
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL129120
Pays : United States
Organisme : NIDDK NIH HHS
ID : U24 DK097771
Pays : United States

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Auteurs

Andrea Arias-Alvarado (A)

Department of Pharmaceutical Sciences, Northeast Ohio Medical University, Rootstown, OH, 44272, USA.

Mirjavid Aghayev (M)

Department of Pharmaceutical Sciences, Northeast Ohio Medical University, Rootstown, OH, 44272, USA.

Serguei Ilchenko (S)

Department of Pharmaceutical Sciences, Northeast Ohio Medical University, Rootstown, OH, 44272, USA.

Nadia Rachdaoui (N)

Department of Animal Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, 08901, USA.

Josephine Lepp (J)

Department of Pharmaceutical Sciences, Northeast Ohio Medical University, Rootstown, OH, 44272, USA.

Tsung-Heng Tsai (TH)

Department of Mathematical Sciences, Kent State University, Kent, OH, 44242, USA.

Guo-Fang Zhang (GF)

Division of Division of Endocrinology, Metabolism and Nutrition, Duke Molecular Physiology Institute, And Department of Medicine, Duke University, Durham, NC, 27701, USA.

Stephen Previs (S)

Merck & Co., Inc, 2000 Galloping Hill Rd, Kenilworth, NJ, 07033, USA.

Takhar Kasumov (T)

Department of Pharmaceutical Sciences, Northeast Ohio Medical University, Rootstown, OH, 44272, USA; Departments of Gastroenterology, Hepatology and Nutrition, Cleveland Clinic Foundation, Cleveland, OH, 44195, USA. Electronic address: tkasumov@neomed.edu.

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Classifications MeSH