Genetic characterization and pathogenicity of a divergent broiler-origin orthoreovirus causing arthritis in China.
avian orthoreovirus (ARV)
complete genome sequencing
isolation
pathogenicity
Journal
Transboundary and emerging diseases
ISSN: 1865-1682
Titre abrégé: Transbound Emerg Dis
Pays: Germany
ID NLM: 101319538
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
revised:
13
11
2020
received:
09
07
2020
accepted:
16
12
2020
pubmed:
21
12
2020
medline:
26
11
2021
entrez:
20
12
2020
Statut:
ppublish
Résumé
Since 2013, there have been an increasing number of cases of arthritis in broilers caused by avian orthoreovirus (ARV) in China, and the virus remains highly virulent in chicks with high-level maternal antibodies. However, little information is available about the complete gene analysis and pathogenicity of the epidemic ARVs. In the study, the ARV strain (V-ARV-SD26) was isolated from broilers associated with arthritis in Shandong Province. To genetically characterize the ARV strain, the whole-genome sequencing was conducted by next-generation sequencing (NGS) technique. Sequence analysis demonstrated that V-ARV-SD26 might have acquired its current genomic composition through several homologous and, in case of the λC, μA and σB, divergent reassortment events. To further investigate the pathogenicity of the strain, 160 one-day-old Ross broilers with maternal antibodies were equally divided into four groups (foodpad-, eye mucosa- and intramuscular-inoculated groups and the negative control group), three experimental groups were inoculated separately with the low-dose virus fluid, and the negative control was equally inoculated with sterile PBS. The results showed that the symptoms of broilers in foodpad inoculation group were the most serious, while that of the eye mucosa infection group were the mildest. Meanwhile, the cloacal cotton swabs and organs were collected for qRT-PCR detection to evaluate the infection status. In conclusion, these findings indicate that V-ARV-SD26 is a divergent ARV strain, which provide experimental data for the prevention and control of newly emerged reovirus, and have a certain reference value for the preparation and evaluation of new vaccines.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3552-3562Subventions
Organisme : Shandong Taishan Industry Leading Talent Project
ID : LJNY201610
Organisme : Funds of Shandong "Double Top" Program
Organisme : National Key Research and Development Program of China
ID : 2018YFD0500106-3
Organisme : Shandong Higher Education Youth Science and Technology Support Program
ID : 2019KJF022
Informations de copyright
© 2020 Wiley-VCH GmbH.
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