Labeling and tracking of immune cells in ex vivo human skin.
Journal
Nature protocols
ISSN: 1750-2799
Titre abrégé: Nat Protoc
Pays: England
ID NLM: 101284307
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
06
03
2020
accepted:
06
10
2020
pubmed:
23
12
2020
medline:
9
3
2021
entrez:
22
12
2020
Statut:
ppublish
Résumé
Human skin harbors various immune cells that are crucial for the control of injury and infection. However, the current understanding of immune cell function within viable human skin tissue is limited. We developed an ex vivo imaging approach in which fresh skin biopsies are mounted and then labeled with nanobodies or antibodies against cell surface markers on tissue-resident memory CD8
Identifiants
pubmed: 33349704
doi: 10.1038/s41596-020-00435-8
pii: 10.1038/s41596-020-00435-8
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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