Diacetoxyscirpenol-induced heterophil extracellular traps contribute to the immune toxicity of liver injury in chickens.
Chicken
Diacetoxyscirpenol
Glycolysis
Heterophils extracellular traps
Liver injury
Journal
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
ISSN: 1873-6351
Titre abrégé: Food Chem Toxicol
Pays: England
ID NLM: 8207483
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
28
08
2020
revised:
20
11
2020
accepted:
11
12
2020
pubmed:
23
12
2020
medline:
22
6
2021
entrez:
22
12
2020
Statut:
ppublish
Résumé
Diacetoxyscirpenol (DAS) is one kind of type A trichothecene mycotoxin which produced by Fusarium species which contaminates agricultural crops and food. DAS attracts particular attention because of the strong toxicity. Heterophil extracellular traps (HETs) is a defense mechanism in the chicken innate immune. In this study, we firstly examine the effects and molecular mechanisms of DAS on HETs release, and then investigate the immune toxicity of DAS-induced HETs on chicken liver. HETs structures were observed by immunofluorescence staining and mechanisms were investigated by fluorescence microplate and Western blot. The results showed DAS triggered HETs formation which consists of chromatin decorated with citrullinated histone 3 (citH3) and elastase. Glycolysis was confirmed to be involved in this process and the inhibitors of NADPH oxidase, ERK
Identifiants
pubmed: 33352262
pii: S0278-6915(20)30816-4
doi: 10.1016/j.fct.2020.111926
pii:
doi:
Substances chimiques
Trichothecenes
0
NADPH Oxidases
EC 1.6.3.-
diacetoxyscirpenol
UYL28I099N
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111926Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.